Electron-nuclear double resonance of horseradish peroxidase compound I. Detection of the porphyrin pi-cation radical.

J. E. Roberts*, B. M. Hoffman, R. Rutter, L. P. Hager

*Corresponding author for this work

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

Electron-nuclear double resonance spectra of horseradish peroxidase Compound I exhibit resonances from 14N and beta-protons, and most probably alpha-protons as well. The measurements prove that the oxyferryl iron of this enzymic intermediate is spin-coupled to a porphyrin radical. Comparison of experimentally obtained average pi-electron spin densities with theoretical predictions for a 4-fold symmetric porphyrin pi-cation radical is supportive of a 2A2u assignment for the radical's electronic state.

Original languageEnglish (US)
Pages (from-to)2118-2121
Number of pages4
JournalJournal of Biological Chemistry
Volume256
Issue number5
StatePublished - Mar 10 1981

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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