Electron Transfer through DNA: Site‐Specific Modification of Duplex DNA with Ruthenium Donors and Acceptors

Thomas J. Meade*, Jon F. Kayyem

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

285 Scopus citations

Abstract

The rate of the electron transfer between two ruthenium centers at the ends of an octanucleotide duplex (metal‐to‐metal distance = 21 Å) was determined to be 1.6(4) × 106 s−1. By using automated DNA synthesis techniques, modified oligonucleotides were prepared with a protected phosphoroamidite of 2′‐amino‐2′‐deoxyuridine. The NH2‐modified oligonucleotides were hybridized with complementary DNA sequences to protect nucleotide bases, and then treated with ruthenium complexes. In this way the ruthenium complex was bound only to the free NH2 group.

Original languageEnglish (US)
Pages (from-to)352-354
Number of pages3
JournalAngewandte Chemie International Edition in English
Volume34
Issue number3
DOIs
StatePublished - Feb 21 1995

Keywords

  • bioinorganic chemistry
  • electron transfer
  • oligonucleotides
  • ruthenium compounds

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Electron Transfer through DNA: Site‐Specific Modification of Duplex DNA with Ruthenium Donors and Acceptors'. Together they form a unique fingerprint.

Cite this