Electrophysiological analysis of neuronal chemokine receptors

Seog Bae Oh, Charlene Cho, Richard J. Miller

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Several studies have shown that neurons in the central and peripheral nervous systems express a variety of chemokine receptors (CKRs). Activation of these receptors can influence neuronal signaling by regulating synaptic transmission and neuronal excitability. This article presents electrophysiological methods that are currently used to study the normal and pathophysiological role for CKRs in the nervous system. Conventional electrophysiological methods such as patch-clamp recording of isolated neurons, brain slices, and heterologous expression systems are described. In addition, single-cell reverse transcription-polymerase chain reaction is discussed as a technique that can be used in conjunction with patch-clamp recording to further investigate the molecular basis of neuronal CKR activation.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalMethods
Volume29
Issue number4
DOIs
StatePublished - Apr 1 2003

Keywords

  • Chemokines
  • Neuronal signaling
  • Patch clamp recording
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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