TY - JOUR
T1 - Electrophysiological assessment of a peptide amphiphile nanofiber nerve graft for facial nerve repair
AU - Greene, Jacqueline J.
AU - McClendon, Mark T.
AU - Stephanopoulos, Nicholas
AU - Álvarez, Zaida
AU - Stupp, Samuel I.
AU - Richter, Claus Peter
N1 - Funding Information:
The authors are especially grateful for the technical support and expertise of Lennell Reynolds (Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center), Dr Lin Li (Mouse Histology and Phenotyping Laboratory, Northwestern University), and Hunter Young (laboratory manager, Richter lab, Northwestern University). Research reported in this publication was supported, in part, by the American Academy of Facial Plastic and Reconstructive Surgery Foundation Leslie Bernstein Grant, and the National Institutes of Health's National Center for Advancing Translational Sciences, Grant Number UL1TR000150, as administered by the Northwestern University Clinical and Translational Sciences Institute (NUCATS) pilot grant program. S. I. S. research was supported by the Northwestern University Center for Regenerative Nanomedicine through a CRN Catalyst Award. Z. A. has received postdoctoral support from Beatriu de Pin?s Fellowship under award # 2014 BP-A 00007 (Ag?ncia de Gesti? d'Ajust Universitaris i de Recerca, AGAUR) and from Paralyzed Veterans of America (PVA) Research Foundation under award # PVA17_RF_0008. N. S. was supported by an NIH Ruth L. Kirschstein NRSA postdoctoral fellowship under award # 1F32NS077728-01A1. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Funding Information:
The authors are especially grateful for the technical support and expertise of Lennell Reynolds (Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center), Dr Lin Li (Mouse Histology and Phenotyping Laboratory, Northwestern University), and Hunter Young (laboratory manager, Richter lab, Northwestern University). Research reported in this publication was supported, in part, by the American Academy of Facial Plastic and Reconstructive Surgery Foundation Leslie Bernstein Grant, and the National Institutes of Health's National Center for Advancing Translational Sciences, Grant Number UL1TR000150, as administered by the Northwestern University Clinical and Translational Sciences Institute (NUCATS) pilot grant program. S. I. S. research was supported by the Northwestern University Center for Regenerative Nanomedicine through a CRN Catalyst Award. Z. A. has received postdoctoral support from Beatriu de Pinós Fellowship under award # 2014 BP‐A 00007 (Agència de Gestió d'Ajust Universitaris i de Recerca, AGAUR) and from Paralyzed Veterans of America (PVA) Research Foundation under award # PVA17_RF_0008. N. S. was supported by an NIH Ruth L. Kirschstein NRSA postdoctoral fellowship under award # 1F32NS077728‐01A1. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Funding Information:
Beatriu de Pinós Fellowship 2014 (Agència de Gestió d'Ajust Universitaris i de Recerca, AGAUR), Grant/Award Number: BP‐A 00007; Northwestern University Clinical and Translational Sciences Institute (NUCATS); North-western University Center for Regenerative Nanomedicine through a CRN Catalyst Award; Paralyzed Veterans of America (PVA) Research Foundation, Grant/Award Number: PVA17_RF_0008; Agència de Gestió d'Ajust Universitaris i de Recerca, AGAUR, Grant/ Award Number: BP‐A 00007; National Institutes of Health's National Center for Advancing Translational Sciences, Grant/Award Number: UL1TR000150; American Academy of Facial Plastic and Reconstructive Surgery Foundation, Grant/Award Number: Leslie Bernstein CORE grant; NIH Ruth L. Kirschstein NRSA, Grant/Award Number: 1F32NS077728‐01A1
Publisher Copyright:
Copyright © 2018 John Wiley & Sons, Ltd.
PY - 2018/6
Y1 - 2018/6
N2 - Facial nerve injury can cause severe long-term physical and psychological morbidity. There are limited repair options for an acutely transected facial nerve not amenable to primary neurorrhaphy. We hypothesize that a peptide amphiphile nanofiber neurograft may provide the nanostructure necessary to guide organized neural regeneration. Five experimental groups were compared, animals with (1) an intact nerve, (2) following resection of a nerve segment, and following resection and immediate repair with either a (3) autograft (using the resected nerve segment), (4) neurograft, or (5) empty conduit. The buccal branch of the rat facial nerve was directly stimulated with charge balanced biphasic electrical current pulses at different current amplitudes whereas nerve compound action potentials (nCAPs) and electromygraphic responses were recorded. After 8 weeks, the proximal buccal branch was surgically reexposed and electrically evoked nCAPs were recorded for groups 1–5. As expected, the intact nerves required significantly lower current amplitudes to evoke an nCAP than those repaired with the neurograft and autograft nerves. For other electrophysiologic parameters such as latency and maximum nCAP, there was no significant difference between the intact, autograft, and neurograft groups. The resected group had variable responses to electrical stimulation, and the empty tube group was electrically silent. Immunohistochemical analysis and transmission electron microscopy confirmed myelinated neural regeneration. This study demonstrates that the neuroregenerative capability of peptide amphiphile nanofiber neurografts is similar to the current clinical gold standard method of repair and holds potential as an off-the-shelf solution for facial reanimation and potentially peripheral nerve repair.
AB - Facial nerve injury can cause severe long-term physical and psychological morbidity. There are limited repair options for an acutely transected facial nerve not amenable to primary neurorrhaphy. We hypothesize that a peptide amphiphile nanofiber neurograft may provide the nanostructure necessary to guide organized neural regeneration. Five experimental groups were compared, animals with (1) an intact nerve, (2) following resection of a nerve segment, and following resection and immediate repair with either a (3) autograft (using the resected nerve segment), (4) neurograft, or (5) empty conduit. The buccal branch of the rat facial nerve was directly stimulated with charge balanced biphasic electrical current pulses at different current amplitudes whereas nerve compound action potentials (nCAPs) and electromygraphic responses were recorded. After 8 weeks, the proximal buccal branch was surgically reexposed and electrically evoked nCAPs were recorded for groups 1–5. As expected, the intact nerves required significantly lower current amplitudes to evoke an nCAP than those repaired with the neurograft and autograft nerves. For other electrophysiologic parameters such as latency and maximum nCAP, there was no significant difference between the intact, autograft, and neurograft groups. The resected group had variable responses to electrical stimulation, and the empty tube group was electrically silent. Immunohistochemical analysis and transmission electron microscopy confirmed myelinated neural regeneration. This study demonstrates that the neuroregenerative capability of peptide amphiphile nanofiber neurografts is similar to the current clinical gold standard method of repair and holds potential as an off-the-shelf solution for facial reanimation and potentially peripheral nerve repair.
KW - electrophysiology
KW - facial nerve repair
KW - nanofiber neurograft
KW - neural regeneration
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U2 - 10.1002/term.2669
DO - 10.1002/term.2669
M3 - Article
C2 - 29701919
AN - SCOPUS:85047663410
SN - 1932-6254
VL - 12
SP - 1389
EP - 1401
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
IS - 6
ER -