Electrophysiological measurement of cannabinoid-mediated synaptic modulation in acute mouse brain slices

Rita Báldi, Dipanwita Ghosh, Brad A. Grueter, Sachin Patel

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Endocannabinoids (eCBs) are a class of bioactive lipids that mediate retrograde synaptic modulation at central and peripheral synapses. The highly lipophilic nature of eCBs and the pharmacological tools available to interrogate this system require unique methodological consideration, especially when applied to ex vivo systems such as electrophysiological analysis in acute brain slices. This unit provides protocols for measuring cannabinoid and eCB-mediated synaptic signaling in mouse brain slices, including analysis of short-term, long-term, and tonic eCB signaling modes, and the unique considerations for working with eCBs and TRPV1/cannabinoid ligands in acute brain slices.

Original languageEnglish (US)
Pages (from-to)6.29.1-6.29.19
JournalCurrent Protocols in Neuroscience
Volume2016
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • Cannabinoid
  • CB1 receptor
  • Electrophysiology
  • Endocannabinoid
  • Mouse

ASJC Scopus subject areas

  • Neuroscience(all)

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