Elevated bladder urine concentration of transforming growth factor-β1 correlates with upper urinary tract obstruction in children

Peter D. Furness*, Max Maizels, Sang Won Han, Richard A Cohn, Earl Y Cheng

*Corresponding author for this work

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Purpose: We evaluated urinary transforming growth factor-β1 (TGF-β1) concentration in children with upper urinary tract obstruction as a potential tool for supporting the diagnosis of clinically significant obstruction. Materials and Methods: Renal pelvic and bladder urine samples were obtained for analysis from 30 patients a median of 5 months old who underwent surgery for obstruction at the ureteropelvic (29) and ureterovesical (1) junctions. Urinary TGF-β1 concentration was measured using a quantitative sandwich enzyme-linked immunoassay technique. Bladder urine TGF-β1 in patients with obstruction was compared with that in controls. In addition, we compared renal pelvic and bladder urine TGF-β1 in patients with obstruction. Results: Mean bladder urine TGF-β1 plus or minus standard error of mean was 4-fold higher in patients with upper tract obstruction than in controls (195 ± 29 versus 47 ± 7 pg./mg. creatinine, p <0.001). In the obstructed group mean TGF-β1 in the renal pelvic urine was 378 ± 86 pg./mg. creatinine, or twice that of the bladder urine (p = 0.02). Conclusions: Bladder urine TGF-β1 in patients with upper urinary tract obstruction is significantly elevated compared with that in controls. To our knowledge our study is the first to identify a bladder urinary marker that correlates with upper urinary tract obstruction with greater than 90% sensitivity. Measuring TGF-β1 in a voided bladder urine sample may provide an objective and noninvasive test for assisting in the diagnosis of upper urinary tract obstruction.

Original languageEnglish (US)
Pages (from-to)1033-1036
Number of pages4
JournalJournal of Urology
Volume162
Issue number3 II
DOIs
StatePublished - Jan 1 1999

Fingerprint

Transforming Growth Factors
Urinary Tract
Urinary Bladder
Urine
Kidney
Creatinine
Immunoenzyme Techniques

Keywords

  • Bladder
  • Cytokines
  • Hydronephrosis
  • Transforming growth factor beta
  • Urine

ASJC Scopus subject areas

  • Urology

Cite this

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title = "Elevated bladder urine concentration of transforming growth factor-β1 correlates with upper urinary tract obstruction in children",
abstract = "Purpose: We evaluated urinary transforming growth factor-β1 (TGF-β1) concentration in children with upper urinary tract obstruction as a potential tool for supporting the diagnosis of clinically significant obstruction. Materials and Methods: Renal pelvic and bladder urine samples were obtained for analysis from 30 patients a median of 5 months old who underwent surgery for obstruction at the ureteropelvic (29) and ureterovesical (1) junctions. Urinary TGF-β1 concentration was measured using a quantitative sandwich enzyme-linked immunoassay technique. Bladder urine TGF-β1 in patients with obstruction was compared with that in controls. In addition, we compared renal pelvic and bladder urine TGF-β1 in patients with obstruction. Results: Mean bladder urine TGF-β1 plus or minus standard error of mean was 4-fold higher in patients with upper tract obstruction than in controls (195 ± 29 versus 47 ± 7 pg./mg. creatinine, p <0.001). In the obstructed group mean TGF-β1 in the renal pelvic urine was 378 ± 86 pg./mg. creatinine, or twice that of the bladder urine (p = 0.02). Conclusions: Bladder urine TGF-β1 in patients with upper urinary tract obstruction is significantly elevated compared with that in controls. To our knowledge our study is the first to identify a bladder urinary marker that correlates with upper urinary tract obstruction with greater than 90{\%} sensitivity. Measuring TGF-β1 in a voided bladder urine sample may provide an objective and noninvasive test for assisting in the diagnosis of upper urinary tract obstruction.",
keywords = "Bladder, Cytokines, Hydronephrosis, Transforming growth factor beta, Urine",
author = "Furness, {Peter D.} and Max Maizels and Han, {Sang Won} and Cohn, {Richard A} and Cheng, {Earl Y}",
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Elevated bladder urine concentration of transforming growth factor-β1 correlates with upper urinary tract obstruction in children. / Furness, Peter D.; Maizels, Max; Han, Sang Won; Cohn, Richard A; Cheng, Earl Y.

In: Journal of Urology, Vol. 162, No. 3 II, 01.01.1999, p. 1033-1036.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated bladder urine concentration of transforming growth factor-β1 correlates with upper urinary tract obstruction in children

AU - Furness, Peter D.

AU - Maizels, Max

AU - Han, Sang Won

AU - Cohn, Richard A

AU - Cheng, Earl Y

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Purpose: We evaluated urinary transforming growth factor-β1 (TGF-β1) concentration in children with upper urinary tract obstruction as a potential tool for supporting the diagnosis of clinically significant obstruction. Materials and Methods: Renal pelvic and bladder urine samples were obtained for analysis from 30 patients a median of 5 months old who underwent surgery for obstruction at the ureteropelvic (29) and ureterovesical (1) junctions. Urinary TGF-β1 concentration was measured using a quantitative sandwich enzyme-linked immunoassay technique. Bladder urine TGF-β1 in patients with obstruction was compared with that in controls. In addition, we compared renal pelvic and bladder urine TGF-β1 in patients with obstruction. Results: Mean bladder urine TGF-β1 plus or minus standard error of mean was 4-fold higher in patients with upper tract obstruction than in controls (195 ± 29 versus 47 ± 7 pg./mg. creatinine, p <0.001). In the obstructed group mean TGF-β1 in the renal pelvic urine was 378 ± 86 pg./mg. creatinine, or twice that of the bladder urine (p = 0.02). Conclusions: Bladder urine TGF-β1 in patients with upper urinary tract obstruction is significantly elevated compared with that in controls. To our knowledge our study is the first to identify a bladder urinary marker that correlates with upper urinary tract obstruction with greater than 90% sensitivity. Measuring TGF-β1 in a voided bladder urine sample may provide an objective and noninvasive test for assisting in the diagnosis of upper urinary tract obstruction.

AB - Purpose: We evaluated urinary transforming growth factor-β1 (TGF-β1) concentration in children with upper urinary tract obstruction as a potential tool for supporting the diagnosis of clinically significant obstruction. Materials and Methods: Renal pelvic and bladder urine samples were obtained for analysis from 30 patients a median of 5 months old who underwent surgery for obstruction at the ureteropelvic (29) and ureterovesical (1) junctions. Urinary TGF-β1 concentration was measured using a quantitative sandwich enzyme-linked immunoassay technique. Bladder urine TGF-β1 in patients with obstruction was compared with that in controls. In addition, we compared renal pelvic and bladder urine TGF-β1 in patients with obstruction. Results: Mean bladder urine TGF-β1 plus or minus standard error of mean was 4-fold higher in patients with upper tract obstruction than in controls (195 ± 29 versus 47 ± 7 pg./mg. creatinine, p <0.001). In the obstructed group mean TGF-β1 in the renal pelvic urine was 378 ± 86 pg./mg. creatinine, or twice that of the bladder urine (p = 0.02). Conclusions: Bladder urine TGF-β1 in patients with upper urinary tract obstruction is significantly elevated compared with that in controls. To our knowledge our study is the first to identify a bladder urinary marker that correlates with upper urinary tract obstruction with greater than 90% sensitivity. Measuring TGF-β1 in a voided bladder urine sample may provide an objective and noninvasive test for assisting in the diagnosis of upper urinary tract obstruction.

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