TY - JOUR
T1 - Elevated expression of the genes for transforming growth factor-β1 and type VI collagen in difuse fasciitis associated with the eosinophiliamyalgia syndrome
AU - Peltonen, Juha
AU - Varga, John
AU - Sollberg, Stephan
AU - Uitto, Jouni
AU - Jimenez, Sergio A.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1991/1
Y1 - 1991/1
N2 - Full-thickness skin biopsies obtained from four patients with rapidly progressive diffuse fasciitis associated with the Eosinophilia-Myalgia syndrome (EMS) were examined for the expression of transforming growth factor-β1(TGF-β1), type VI collagen, and fibronectin genes employing immunohisto chemistry and in situ hybridizations. The immunohistochemical studies demonstrated increased deposition of TGF-β1 type VI collagen, and fibronectin epitopes in the extracellular matrix of the fascia in comparison to the adjacent dermis in the same specimens. Increased levels of type VI collagen mRNA, as evidenced by positive in situ hybridization signals with an α2(VI) collagen cDNA, were also found in the fascia in comparison with the dermis. In situ hybridizations of affected fascia with a human sequence-specific TGF-β1 cDNA demonstrated numerous fibroblasts displaying positive hybridization signals indicative of high levels of transcripts for this cytokine. In contrast, no hybridization signal for TGF-β1 was detected in fibroblasts in the adjacent dermis. These findings suggest that TGF β1 may play an important role in the development of the connective tissue alterations present in EMS-associated diffuse fasciitis.
AB - Full-thickness skin biopsies obtained from four patients with rapidly progressive diffuse fasciitis associated with the Eosinophilia-Myalgia syndrome (EMS) were examined for the expression of transforming growth factor-β1(TGF-β1), type VI collagen, and fibronectin genes employing immunohisto chemistry and in situ hybridizations. The immunohistochemical studies demonstrated increased deposition of TGF-β1 type VI collagen, and fibronectin epitopes in the extracellular matrix of the fascia in comparison to the adjacent dermis in the same specimens. Increased levels of type VI collagen mRNA, as evidenced by positive in situ hybridization signals with an α2(VI) collagen cDNA, were also found in the fascia in comparison with the dermis. In situ hybridizations of affected fascia with a human sequence-specific TGF-β1 cDNA demonstrated numerous fibroblasts displaying positive hybridization signals indicative of high levels of transcripts for this cytokine. In contrast, no hybridization signal for TGF-β1 was detected in fibroblasts in the adjacent dermis. These findings suggest that TGF β1 may play an important role in the development of the connective tissue alterations present in EMS-associated diffuse fasciitis.
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U2 - 10.1111/1523-1747.ep12514683
DO - 10.1111/1523-1747.ep12514683
M3 - Article
C2 - 1702819
AN - SCOPUS:0026016044
VL - 96
SP - 20
EP - 25
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 1
ER -