Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis

J. A. Poposki, S. Peterson, K. Welch, R. P. Schleimer, K. E. Hulse, A. T. Peters, J. Norton, L. A. Suh, R. Carter, K. E. Harris, L. C. Grammer, B. K. Tan, R. K. Chandra, D. B. Conley, R. C. Kern, A. Kato*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Summary: Background: Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen-presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied. Objective: The objective of this study was to characterize DC subsets in CRS. Methods: We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry. Results: Compared to UT from control subjects (n = 15) and patients with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c+ cells but not CD303+ cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a+ cells in NPs might be a subset of mDC1s and that CD45+CD19-CD1c+CD11c+CD141-CD303-HLA-DR+ mDC1s and CD45+CD19-CD11c+CD1c-CD141high HLA-DR+ mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45+CD11c-CD303+HLA-DR+ pDCs were only elevated in NPs compared to control UT. Conclusion and Clinical Relevance: Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP.

Original languageEnglish (US)
Pages (from-to)384-393
Number of pages10
JournalClinical and Experimental Allergy
Volume45
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Nasal Polyps
Myeloid Cells
Dendritic Cells
HLA-DR Antigens
Inflammation
Messenger RNA
Immunohistochemistry
Langerhans Cells
Adaptive Immunity
Antigen-Presenting Cells

Keywords

  • Chronic rhinosinusitis
  • Myeloid dendritic cells
  • Nasal polyps
  • Plasmacytoid dendritic cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

@article{3366f7727cd648b688fce840f0e687a3,
title = "Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis",
abstract = "Summary: Background: Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen-presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied. Objective: The objective of this study was to characterize DC subsets in CRS. Methods: We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry. Results: Compared to UT from control subjects (n = 15) and patients with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c+ cells but not CD303+ cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a+ cells in NPs might be a subset of mDC1s and that CD45+CD19-CD1c+CD11c+CD141-CD303-HLA-DR+ mDC1s and CD45+CD19-CD11c+CD1c-CD141high HLA-DR+ mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45+CD11c-CD303+HLA-DR+ pDCs were only elevated in NPs compared to control UT. Conclusion and Clinical Relevance: Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP.",
keywords = "Chronic rhinosinusitis, Myeloid dendritic cells, Nasal polyps, Plasmacytoid dendritic cells",
author = "Poposki, {J. A.} and S. Peterson and K. Welch and Schleimer, {R. P.} and Hulse, {K. E.} and Peters, {A. T.} and J. Norton and Suh, {L. A.} and R. Carter and Harris, {K. E.} and Grammer, {L. C.} and Tan, {B. K.} and Chandra, {R. K.} and Conley, {D. B.} and Kern, {R. C.} and A. Kato",
year = "2015",
month = "2",
day = "1",
doi = "10.1111/cea.12471",
language = "English (US)",
volume = "45",
pages = "384--393",
journal = "Clinical and Experimental Allergy",
issn = "0954-7894",
publisher = "Wiley-Blackwell",
number = "2",

}

Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis. / Poposki, J. A.; Peterson, S.; Welch, K.; Schleimer, R. P.; Hulse, K. E.; Peters, A. T.; Norton, J.; Suh, L. A.; Carter, R.; Harris, K. E.; Grammer, L. C.; Tan, B. K.; Chandra, R. K.; Conley, D. B.; Kern, R. C.; Kato, A.

In: Clinical and Experimental Allergy, Vol. 45, No. 2, 01.02.2015, p. 384-393.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis

AU - Poposki, J. A.

AU - Peterson, S.

AU - Welch, K.

AU - Schleimer, R. P.

AU - Hulse, K. E.

AU - Peters, A. T.

AU - Norton, J.

AU - Suh, L. A.

AU - Carter, R.

AU - Harris, K. E.

AU - Grammer, L. C.

AU - Tan, B. K.

AU - Chandra, R. K.

AU - Conley, D. B.

AU - Kern, R. C.

AU - Kato, A.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Summary: Background: Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen-presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied. Objective: The objective of this study was to characterize DC subsets in CRS. Methods: We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry. Results: Compared to UT from control subjects (n = 15) and patients with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c+ cells but not CD303+ cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a+ cells in NPs might be a subset of mDC1s and that CD45+CD19-CD1c+CD11c+CD141-CD303-HLA-DR+ mDC1s and CD45+CD19-CD11c+CD1c-CD141high HLA-DR+ mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45+CD11c-CD303+HLA-DR+ pDCs were only elevated in NPs compared to control UT. Conclusion and Clinical Relevance: Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP.

AB - Summary: Background: Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen-presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied. Objective: The objective of this study was to characterize DC subsets in CRS. Methods: We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry. Results: Compared to UT from control subjects (n = 15) and patients with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c+ cells but not CD303+ cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a+ cells in NPs might be a subset of mDC1s and that CD45+CD19-CD1c+CD11c+CD141-CD303-HLA-DR+ mDC1s and CD45+CD19-CD11c+CD1c-CD141high HLA-DR+ mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45+CD11c-CD303+HLA-DR+ pDCs were only elevated in NPs compared to control UT. Conclusion and Clinical Relevance: Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP.

KW - Chronic rhinosinusitis

KW - Myeloid dendritic cells

KW - Nasal polyps

KW - Plasmacytoid dendritic cells

UR - http://www.scopus.com/inward/record.url?scp=84923090084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923090084&partnerID=8YFLogxK

U2 - 10.1111/cea.12471

DO - 10.1111/cea.12471

M3 - Article

VL - 45

SP - 384

EP - 393

JO - Clinical and Experimental Allergy

JF - Clinical and Experimental Allergy

SN - 0954-7894

IS - 2

ER -