Elution of kidney-bound antibody by ph-shift does not discriminate between antibodies differing in functional affinity for their ligand

Winston Y. Shih, Michael D. Clayman, Carolyn J. Kelly, William Hines, Eric G. Neilson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Antibodies eluted from kidneys by traditional methods of pH shift have been used as reagents in a wide variety of experimental analyses without knowledge of whether their ligand affinity influenced their re-moval from parenchymal tissue. In the current study we employed two monoclonal antibodies, differing only in their functional affinity (high; K = 2.1 x 108/Mand low; K = 6.2 x 106/M) to a common ligand found on the renal basement membrane, to evaluate whether a standard elution technique might selectively facilitate the removal of one antibody over the other. Our findings indicate, however, that the routine methods of elution by pH shift remove both antibodies equally well (41-48%), and without loss of paratypic function. These results provide new evidence that elution by pH shift can produce eluate antibodies which are not biased by preferred affinities.

Original languageEnglish (US)
Pages (from-to)329-333
Number of pages5
JournalInternational archives of allergy and immunology
Volume87
Issue number3
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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