Embedding

Robert Tagliaferro; Brandon Possum; Steven Wilson; Anastasia Dukhnina; Frances Walocko; Murad Alam; Kishwer S. Nehal; Camille McKay

doi:10.1007/978-3-031-52434-9_4

Embedding

Robert Tagliaferro, Brandon Possum, Steven Wilson, Anastasia Dukhnina, Frances Walocko, Murad Alam, Kishwer S. Nehal^*, Camille McKay

^*Corresponding author for this work

Dermatology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Embedding is the process of fixing a flattened tissue specimen in embedding media. Accurate embedding of Mohs tissue specimens is essential to preparing the entire peripheral and deep surgical margin for high-quality Mohs tissue sections. Multiple varied techniques for embedding Mohs tissue specimens and related equipment Equipment are described in detail. Potential pitfalls encountered during the embedding process are highlighted, and important troubleshooting Troubleshooting tips are outlined.

Original language	English (US)
Title of host publication	Laboratory Manual for Mohs Micrographic Surgery
Subtitle of host publication	Frozen Tissue Processing
Publisher	Springer International Publishing
Pages	27-49
Number of pages	23
ISBN (Electronic)	9783031524349
ISBN (Print)	9783031524332
DOIs	https://doi.org/10.1007/978-3-031-52434-9_4
State	Published - Apr 18 2024

Keywords

Cryostat stage method
Embedding wells method
Floating tissue method
Glass slide method
Heat extractor surface method
Optimum Cutting Temperature (OCT) embedding media
Quality control
Reverse heat extractor method
Tissue relaxation
Whole mount method

ASJC Scopus subject areas

General Medicine

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10.1007/978-3-031-52434-9_4

Cite this

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title = "Embedding",

abstract = "Embedding is the process of fixing a flattened tissue specimen in embedding media. Accurate embedding of Mohs tissue specimens is essential to preparing the entire peripheral and deep surgical margin for high-quality Mohs tissue sections. Multiple varied techniques for embedding Mohs tissue specimens and related equipment Equipment are described in detail. Potential pitfalls encountered during the embedding process are highlighted, and important troubleshooting Troubleshooting tips are outlined.",

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author = "Robert Tagliaferro and Brandon Possum and Steven Wilson and Anastasia Dukhnina and Frances Walocko and Murad Alam and Nehal, {Kishwer S.} and Camille McKay",

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day = "18",

doi = "10.1007/978-3-031-52434-9_4",

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AU - Tagliaferro, Robert

AU - Possum, Brandon

AU - Wilson, Steven

AU - Dukhnina, Anastasia

AU - Walocko, Frances

AU - Alam, Murad

AU - Nehal, Kishwer S.

AU - McKay, Camille

PY - 2024/4/18

Y1 - 2024/4/18

N2 - Embedding is the process of fixing a flattened tissue specimen in embedding media. Accurate embedding of Mohs tissue specimens is essential to preparing the entire peripheral and deep surgical margin for high-quality Mohs tissue sections. Multiple varied techniques for embedding Mohs tissue specimens and related equipment Equipment are described in detail. Potential pitfalls encountered during the embedding process are highlighted, and important troubleshooting Troubleshooting tips are outlined.

AB - Embedding is the process of fixing a flattened tissue specimen in embedding media. Accurate embedding of Mohs tissue specimens is essential to preparing the entire peripheral and deep surgical margin for high-quality Mohs tissue sections. Multiple varied techniques for embedding Mohs tissue specimens and related equipment Equipment are described in detail. Potential pitfalls encountered during the embedding process are highlighted, and important troubleshooting Troubleshooting tips are outlined.

KW - Cryostat stage method

KW - Embedding wells method

KW - Floating tissue method

KW - Glass slide method

KW - Heat extractor surface method

KW - Optimum Cutting Temperature (OCT) embedding media

KW - Quality control

KW - Reverse heat extractor method

KW - Tissue relaxation

KW - Whole mount method

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SP - 27

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BT - Laboratory Manual for Mohs Micrographic Surgery

PB - Springer International Publishing

ER -

Embedding

Abstract

Keywords

ASJC Scopus subject areas

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