Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease

P. Bhattacharya, J. R. Moskal, S. Basu

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Activity of a trypsin sensitive acid deoxyribonuclease (DNase) inhibitor has been detected in 13 to 21 day old embryonic chicken brains and in clonal lines of neuroblastoma cells (adrenergic, N1E 115, and neurotransmitter inactive, N 18) grown for 48 hr after subculture. The activities of purified porcine and bovine spleen DNases were inhibited 60-75% in the presence of the inhibitor, whereas less than 10% inhibition was observed with purified pancreatic DNase. Activities of an acidic DNase and its inhibitor reached maxima in 21 day old embryonic chicken brain. The proteins were separated by isoelectric focusing and affinity column chromatographic techniques. The pI values of the acid DNase and its inhibitor were 5.4 and 4.2, respectively.

Original languageEnglish (US)
Pages (from-to)842-845
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number3
DOIs
StatePublished - 1977

ASJC Scopus subject areas

  • General

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