Embryonic expression and extracellular secretion of Xenopus Slit

J. H. Chen, W. Wu, H. S. Li, T. Fagaly, L. Zhou, J. Y. Wu, Y. Rao*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The slit genes have recently been found to encode proteins with a conserved chemorepulsive activity for axons in invertebrates and vertebrates. We have determined the expression pattern of a slit gene in Xenopus embryos. In the neural tube, slit is expressed at the ventral and dorsal midlines, and the motor neurons. slit is also expressed in a changing pattern in the retina. The full-length Xenopus Slit protein is secreted extracellularly, whereas its receptor Roundabout can not be secreted. Using a myc-tagged secreted Slit protein, we confirmed the binding of Slit to Roundabout expressed on the cell surface.These results confirm Slit-Roundabout interactions and the biochemical properties of Slit and Roundabout proteins, and further support the idea that Slit may guide axon projections in multiple regions of the embryo. Copyright (C) 1999 IBRO.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalNeuroscience
Volume96
Issue number1
DOIs
StatePublished - Jan 2000

Keywords

  • Axon guidance
  • Chemorepulsion
  • Olfactory bulb
  • Roundabout
  • Slit

ASJC Scopus subject areas

  • Neuroscience(all)

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    Chen, J. H., Wu, W., Li, H. S., Fagaly, T., Zhou, L., Wu, J. Y., & Rao, Y. (2000). Embryonic expression and extracellular secretion of Xenopus Slit. Neuroscience, 96(1), 231-236. https://doi.org/10.1016/S0306-4522(99)00408-X