End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis

Yolanda Stypula-Cyrus; Nikhil N. Mutyal; Mart Dela Cruz; Dhananjay P. Kunte; Andrew J. Radosevich; Ramesh Wali; Hemant K. Roy; Vadim Backman

doi:10.1016/j.febslet.2014.01.046

End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis

Yolanda Stypula-Cyrus, Nikhil N. Mutyal, Mart Dela Cruz, Dhananjay P. Kunte, Andrew J. Radosevich, Ramesh Wali, Hemant K. Roy^*, Vadim Backman

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ("field effect") of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.

Original language	English (US)
Pages (from-to)	829-835
Number of pages	7
Journal	FEBS Letters
Volume	588
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2014.01.046
State	Published - Mar 3 2014

Funding

This work was supported by NIH Grants U01CA111257 , R01CA128641 , R01CA165309 , and R01CA156186 .

Keywords

Adenomatous polyposis coli
Colon cancer
End-binding protein 1
Field carcinogenesis
Sub-diffractional structure

ASJC Scopus subject areas

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2014.01.046

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title = "End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis",

abstract = "End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ({"}field effect{"}) of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.",

keywords = "Adenomatous polyposis coli, Colon cancer, End-binding protein 1, Field carcinogenesis, Sub-diffractional structure",

author = "Yolanda Stypula-Cyrus and Mutyal, {Nikhil N.} and {Dela Cruz}, Mart and Kunte, {Dhananjay P.} and Radosevich, {Andrew J.} and Ramesh Wali and Roy, {Hemant K.} and Vadim Backman",

note = "Funding Information: This work was supported by NIH Grants U01CA111257 , R01CA128641 , R01CA165309 , and R01CA156186 .",

year = "2014",

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doi = "10.1016/j.febslet.2014.01.046",

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T1 - End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis

AU - Stypula-Cyrus, Yolanda

AU - Mutyal, Nikhil N.

AU - Dela Cruz, Mart

AU - Kunte, Dhananjay P.

AU - Radosevich, Andrew J.

AU - Wali, Ramesh

AU - Roy, Hemant K.

AU - Backman, Vadim

N1 - Funding Information: This work was supported by NIH Grants U01CA111257 , R01CA128641 , R01CA165309 , and R01CA156186 .

PY - 2014/3/3

Y1 - 2014/3/3

N2 - End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ("field effect") of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.

AB - End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ("field effect") of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.

KW - Adenomatous polyposis coli

KW - Colon cancer

KW - End-binding protein 1

KW - Field carcinogenesis

KW - Sub-diffractional structure

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JO - FEBS Letters

JF - FEBS Letters

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End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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