ENDOGENOUS LEVELS OF NEUROTRANSMITTER CANDIDATES IN PHOTORECEPTOR CELLS OF THE TURTLE RETINA

P. Vijay Sarthy*, Dominic M K Lam

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The neurotransmitter used by vertebrate photoreceptors has not been identified, although aspartic acid, glutamic acid and acetylcholine have all been suggested as possible candidates. In the present study, we have measured the endogenous levels of these substances in isolated photoreceptors of the turtle retina. Fractions containing over 80% of identified photoreceptors were obtained by enzymatic dissociation of the retina followed by velocity sedimentation at unit gravity. The endogenous levels of free amino acids in these fractions were measured by amino acid analysis and the ACh content was measured by a radiochemical assay. In addition, identified photoreceptors were drawn into a micropipet individually under visual observation and their ACh content was determined. Our results show that while the concentrations of free aspartic acid and glutamic acid in isolated photoreceptors are similar to those found in the retina, the concentration of ACh in cone photoreceptors (0.1–0.2 mM) is 2‐ to 3‐fold higher than that in the turtle retina. This result combined with the findings of others suggests that turtle cone photoreceptors may be cholinergic

Original languageEnglish (US)
Pages (from-to)455-461
Number of pages7
JournalJournal of Neurochemistry
Volume32
Issue number2
DOIs
StatePublished - Jan 1 1979

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Photoreceptor Cells
Turtles
Aspartic Acid
Neurotransmitter Agents
Retina
Cones
Glutamic Acid
Retinal Cone Photoreceptor Cells
Amino Acids
Sedimentation
Cholinergic Agents
Acetylcholine
Assays
Gravitation
Vertebrate Photoreceptor Cells
Observation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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title = "ENDOGENOUS LEVELS OF NEUROTRANSMITTER CANDIDATES IN PHOTORECEPTOR CELLS OF THE TURTLE RETINA",
abstract = "The neurotransmitter used by vertebrate photoreceptors has not been identified, although aspartic acid, glutamic acid and acetylcholine have all been suggested as possible candidates. In the present study, we have measured the endogenous levels of these substances in isolated photoreceptors of the turtle retina. Fractions containing over 80{\%} of identified photoreceptors were obtained by enzymatic dissociation of the retina followed by velocity sedimentation at unit gravity. The endogenous levels of free amino acids in these fractions were measured by amino acid analysis and the ACh content was measured by a radiochemical assay. In addition, identified photoreceptors were drawn into a micropipet individually under visual observation and their ACh content was determined. Our results show that while the concentrations of free aspartic acid and glutamic acid in isolated photoreceptors are similar to those found in the retina, the concentration of ACh in cone photoreceptors (0.1–0.2 mM) is 2‐ to 3‐fold higher than that in the turtle retina. This result combined with the findings of others suggests that turtle cone photoreceptors may be cholinergic",
author = "Sarthy, {P. Vijay} and Lam, {Dominic M K}",
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ENDOGENOUS LEVELS OF NEUROTRANSMITTER CANDIDATES IN PHOTORECEPTOR CELLS OF THE TURTLE RETINA. / Sarthy, P. Vijay; Lam, Dominic M K.

In: Journal of Neurochemistry, Vol. 32, No. 2, 01.01.1979, p. 455-461.

Research output: Contribution to journalArticle

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