Endosomal Organization of CpG Constructs Correlates with Enhanced Immune Activation

Kwahun Lee, Ziyin N. Huang, Chad A. Mirkin, Teri W. Odom*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


This Letter describes how the endosomal organization of immunostimulatory nanoconstructs can tune the in vitro activation of macrophages. Nanoconstructs composed of gold nanoparticles conjugated with unmethylated cytosine-phosphate-guanine (CpG) oligonucleotides have distinct endosomal distributions depending on the surface curvature. Mixed-curvature constructs produce a relatively high percentage of hollow endosomes, where constructs accumulated primarily along the interior edges. These constructs achieved a higher level of toll-like receptor (TLR) 9 activation along with the enhanced secretion of proinflammatory cytokines and chemokines compared to constant-curvature constructs that aggregated mostly in the center of the endosomes. Our results underscore the importance of intraendosomal interactions in regulating immune responses and targeted delivery.

Original languageEnglish (US)
Pages (from-to)6170-6175
Number of pages6
JournalNano letters
Issue number8
StatePublished - Aug 12 2020


  • CpG
  • immunostimulation
  • intracellular targeting
  • nanoparticle
  • surface curvature

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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