Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells

Wei Zhang, Noboru Sakai, Hiromu Yamada, Tao Fu, Yoshinori Nozawa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.

Original languageEnglish (US)
Pages (from-to)199-204
Number of pages6
JournalNeuroscience Letters
Volume112
Issue number2-3
DOIs
StatePublished - May 4 1990

Keywords

  • Endothelin-1
  • Glioma cell
  • Inositol 1,4,5-trisphosphate
  • Intracellular calcium

ASJC Scopus subject areas

  • Neuroscience(all)

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