TY - JOUR
T1 - Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells
AU - Zhang, Wei
AU - Sakai, Noboru
AU - Yamada, Hiromu
AU - Fu, Tao
AU - Nozawa, Yoshinori
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. We thank Dr. H. Hirayama and Dr. A. Hara for kindly providing C6 and A-172 cells.
PY - 1990/5/4
Y1 - 1990/5/4
N2 - The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.
AB - The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.
KW - Endothelin-1
KW - Glioma cell
KW - Inositol 1,4,5-trisphosphate
KW - Intracellular calcium
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U2 - 10.1016/0304-3940(90)90203-L
DO - 10.1016/0304-3940(90)90203-L
M3 - Article
C2 - 2193256
AN - SCOPUS:0025342439
SN - 0304-3940
VL - 112
SP - 199
EP - 204
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2-3
ER -