Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells

Wei Zhang; Noboru Sakai; Hiromu Yamada; Tao Fu; Yoshinori Nozawa

doi:10.1016/0304-3940(90)90203-L

Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells

Wei Zhang, Noboru Sakai, Hiromu Yamada, Tao Fu, Yoshinori Nozawa^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca²⁺]_i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP₃(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.

Original language	English (US)
Pages (from-to)	199-204
Number of pages	6
Journal	Neuroscience Letters
Volume	112
Issue number	2-3
DOIs	https://doi.org/10.1016/0304-3940(90)90203-L
State	Published - May 4 1990

Funding

This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. We thank Dr. H. Hirayama and Dr. A. Hara for kindly providing C6 and A-172 cells.

Keywords

Endothelin-1
Glioma cell
Inositol 1,4,5-trisphosphate
Intracellular calcium

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(90)90203-L

Cite this

@article{dcb415f81b1e439bb11c07d5739b8558,

title = "Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells",

abstract = "The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.",

keywords = "Endothelin-1, Glioma cell, Inositol 1,4,5-trisphosphate, Intracellular calcium",

author = "Wei Zhang and Noboru Sakai and Hiromu Yamada and Tao Fu and Yoshinori Nozawa",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. We thank Dr. H. Hirayama and Dr. A. Hara for kindly providing C6 and A-172 cells.",

year = "1990",

month = may,

day = "4",

doi = "10.1016/0304-3940(90)90203-L",

language = "English (US)",

volume = "112",

pages = "199--204",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

}

TY - JOUR

T1 - Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells

AU - Zhang, Wei

AU - Sakai, Noboru

AU - Yamada, Hiromu

AU - Fu, Tao

AU - Nozawa, Yoshinori

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. We thank Dr. H. Hirayama and Dr. A. Hara for kindly providing C6 and A-172 cells.

PY - 1990/5/4

Y1 - 1990/5/4

N2 - The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.

AB - The effects of endothelin-1 (ET) on the signal transduction in rat and human glioma cell line cells have been investigated. ET was found to initiate the increase of intracellular calcium ([Ca2+]i) levels in both C6 and A-172 cells, which was concurrent with the formation of inositol 1,4,5-trisphosphate (IP3(1,4,5)). In the presence of [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) in the incubation media, the duration of the intracellular calcium response was reduced, indicating that the ET-induced increase of intracellular calcium in glioma cells may be mediated by a dual mechanism, intracellular calcium mobilization and influx of extracellular calcium. These results suggest that ET may also act as a neuropeptide in the central nervous system.

KW - Endothelin-1

KW - Glioma cell

KW - Inositol 1,4,5-trisphosphate

KW - Intracellular calcium

UR - https://www.scopus.com/pages/publications/0025342439

UR - https://www.scopus.com/inward/citedby.url?scp=0025342439&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(90)90203-L

DO - 10.1016/0304-3940(90)90203-L

M3 - Article

C2 - 2193256

AN - SCOPUS:0025342439

SN - 0304-3940

VL - 112

SP - 199

EP - 204

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

Endothelin-1 induces intracellular calcium rise and inositol 1,4,5-trisphosphate formation in cultured rat and human glioma cells

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this