Energy-dispersive diffraction tomography of shark vertebral centra

Jun Sang Park, Ryan M. Horn, Haiyan Chen, Kelsey C. James, Michelle S. Passerotti, Lisa J. Natanson, Stuart R. Stock*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Shark vertebrae and their centra (vertebral bodies) are high-performance structures able to survive millions of cycles of high amplitude strain despite lacking a repair mechanism for accumulating damage. Shark centra consist of mineralized cartilage, a biocomposite of bioapatite (bAp), and collagen, and the nanocrystalline bAp's contribution to functionality remains largely uninvestigated. Using the multiple detector energy-dispersive diffraction (EDD) system at 6-BM-B, the Advanced Photon Source, and 3D tomographic sampling, the 3D functionality of entire centra were probed. Immersion in ethanol vs phosphate-buffered saline produces only small changes in bAp d-spacing within a great hammerhead centrum. EDD mapping under in situ loading was performed an entire blue shark centrum, and 3D maps of bAp strain showed the two structural zones of the centrum, the corpus calcareum and intermedialia, contained opposite-signed strains approaching 0.5%, and application of ∼8% nominal strain did not alter these strain magnitudes and their spatial distribution.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalPowder Diffraction
Volume39
Issue number2
DOIs
StatePublished - Jun 1 2024

Funding

This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. Argonne National Laboratory's work was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under contract DE-AC02-06CH11357.

Keywords

  • bioapatite
  • energy-dispersive diffraction
  • shark
  • synchrotron X-radiation
  • tomography
  • vertebral centrum

ASJC Scopus subject areas

  • Radiation
  • General Materials Science
  • Instrumentation
  • Condensed Matter Physics

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