Energy metabolism, replicative ability, intracellular calcium concentration, and ionic channels of horse articular chondrocytes

Franco Vittur*, Micaela Grandolfo, Emanuela Fragonas, Cristiana Godeas, Sergio Paoletti, Piero Pollesello, Bjarne J. Kvam, Fabio Ruzzier, Tanja Starc, Jerzy W. Mozrzymas, Marco Martina, Benedetto De Bernard

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Some aspects of the physiology of chondrocytes from horse articular cartilage were studied, since this animal model can be helpful in understanding arthritic processes. The replicative ability of articular chondrocytes, measured by the incorporation of [3H]thymidine, and their capacity of proteoglycan production, evaluated from the incorporation of [35S]sulfate, are very low. In addition, these cells do not differentiate in vitro as shown by the constant specific activity of alkaline phosphatase measured at different times in culture. Two types of potassium channels were identified by patch clamp experiments in the cell-attached configuration, one characterized by a conductance of 40 pS and the other of 100 pS. No active K+ channels were found at V(pip) = 0. It was shown by Fura-2 experiments that the low replicative ability is paralleled by a modest variation of the intracellular calcium concentration after a mitogenic stimulus. 31P NMR experiments, both on slices of whole articular cartilage and on isolated cells, demonstrate that chondrocytes derive their energy mainly from the glycolytic pathway.

Original languageEnglish (US)
Pages (from-to)130-136
Number of pages7
JournalExperimental Cell Research
Volume210
Issue number1
DOIs
StatePublished - Jan 1994

ASJC Scopus subject areas

  • Cell Biology

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