@article{f77ab2197865475493ffa686ecfb425a,
title = "Engineered ribosomes for basic science and synthetic biology",
abstract = "The ribosome is the cell's factory for protein synthesis. With protein synthesis rates of up to 20 amino acids per second and at an accuracy of 99.99%, the extraordinary catalytic capacity of the bacterial translation machinery has attracted extensive efforts to engineer, reconstruct, and repurpose it for biochemical studies and novel functions. Despite these efforts, the potential for harnessing the translation apparatus to manufacture bio-based products beyond natural limits remains underexploited, and fundamental constraints on the chemistry that the ribosome's RNA-based active site can carry out are unknown. This review aims to cover the past and present advances in ribosome design and engineering to understand the fundamental biology of the ribosome to facilitate the construction of synthetic manufacturing machines. The prospects for the development of engineered, or designer, ribosomes for novel polymer synthesis are reviewed, future challenges are considered, and promising advances in a variety of applications are discussed.",
keywords = "Cell-free, Engineering, Peptides, Ribosome, Tethered ribosome, Translation",
author = "D'Aquino, {Anne E.} and Kim, {Do Soon} and Jewett, {Michael C.}",
note = "Funding Information: We extend a special thanks to our collaborators for thoughtful insight into and editing of this manuscript. This work was supported by the Army Research Office Grant W911NF-16-1-0372 (to M.C.J.), National Science Foundation (NSF) grants MCB-1413563 and MCB-1716766 (to M.C.J.), the Air Force Research Laboratory Center of Excellence Grant FA8650-15-2-5518 (to M.C.J.), the Chicago Biomedical Consortium with support from the Searle Funds at the Chicago Community Trust, the Defense Threat Reduction Agency Grant HDTRA1-15-10052/P00001 (to M.C.J.), the David and Lucile Packard Foundation (to M.C.J.), and the Camille Dreyfus Teacher-Scholar Program (to M.C.J.). A.E.d{\textquoteright}A. and D.S.K. are supported by NSF Graduate Research Fellowships. The US Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright notation thereon. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of the Air Force Research Laboratory, Air Force Office of Scientific Research, Defense Threat Reduction Agency, or US Government. Publisher Copyright: {\textcopyright} 2018 by Annual Reviews. All rights reserved.",
year = "2018",
doi = "10.1146/annurev-chembioeng-060817-084129",
language = "English (US)",
volume = "9",
pages = "311--340",
journal = "Annual Review of Chemical and Biomolecular Engineering",
issn = "1947-5438",
publisher = "Annual Reviews Inc.",
}