Engrailed, Suppressor of fused and Roadkill modulate the Drosophila GLI transcription factor Cubitus interruptus at multiple levels

Nicole Roberto, Isabelle Becam, Anne Plessis, Robert A. Holmgren

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Morphogen gradients need to be robust, but may also need to be tailored for specific tissues. Often this type of regulation is carried out by negative regulators and negative feedback loops. In the Hedgehog (Hh) pathway, activation of patched (ptc) in response to Hh is part of a negative feedback loop limiting the range of the Hh morphogen. Here, we show that in the Drosophila wing imaginal disc two other known Hh targets genes feed back to modulate Hh signaling. First, anterior expression of the transcriptional repressor Engrailed modifies the Hh gradient by attenuating the expression of the Hh pathway transcription factor cubitus interruptus (ci), leading to lower levels of ptc expression. Second, the E-3 ligase Roadkill shifts the competition between the full-length activator and truncated repressor forms of Ci by preferentially targeting full-length Ci for degradation. Finally, we provide evidence that Suppressor of fused, a negative regulator of Hh signaling, has an unexpected positive role, specifically protecting full-length Ci but not the Ci repressor from Roadkill.

Original languageEnglish (US)
JournalDevelopment (Cambridge, England)
Volume149
Issue number6
DOIs
StatePublished - Mar 15 2022

Keywords

  • Drosophila
  • Hedgehog
  • Pathway modulation
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Engrailed, Suppressor of fused and Roadkill modulate the Drosophila GLI transcription factor Cubitus interruptus at multiple levels'. Together they form a unique fingerprint.

Cite this