Enhanced expression of αv integrin subunit and osteopontin during differentiation of HL-60 cells along the monocytic pathway

Martha J. Somerman*, Janice E. Berry, Zhila Khalkhali-Ellis, Philip Osdoby, Robert U. Simpson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

HL-60 cells, a promyelocytic leukemic cell line, provide a good model for studying the role of adhesion molecules and associated receptors involved in cell differentiation. When exposed to factors such as phorbol esters, these cells grown in suspension differentiate into monocytes and adhere to tissue culture dishes. In this study we showed that HL-60 cells exposed to phorbol esters express osteopontin (OPN), a cell adhesion molecule linked with osteoclast function. Moreover, the timed expression of OPN, in phorbol ester treated cells, was linked to increased cell adhesion. Subsequent to the expression of OPN, an increase in mRNA levels for αv integrin subunit was observed. The αvβ3 integrin, a cell surface receptor found in high concentrations in osteoclasts, is considered to be a receptor for OPN. Furthermore, during differentiation we detected an increase in two cell surface markers specific for osteoclasts, 75B and 121F. This is the first report to demonstrate expression of OPN during differentiation of HL-60 cells, indicating that HL-60 cells can be used as a tool to enhance our understanding as to the role of OPN in cell differentiation.

Original languageEnglish (US)
Pages (from-to)335-341
Number of pages7
JournalExperimental Cell Research
Volume216
Issue number2
DOIs
StatePublished - Feb 1995

ASJC Scopus subject areas

  • Cell Biology

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