@article{8188cb44fdc04d349ec2c9dbf3ff3b68,
title = "Enhanced growth of mice lacking the cyclin-dependent kinase inhibitor function of P27Kip1",
abstract = "Disruption of the cyclin-dependent kinase-inhibitory domain of p27 enhances growth of mice. Growth is attributed to an increase in cell number, due to increased cell proliferation, most obviously in tissues that ordinarily express p27 at the highest levels. Disruption of p27 function leads to nodular hyperplasia in the intermediate lobe of the pituitary. However, increased growth occurs without an increase in the amounts of either growth hormone or IGF-I. In addition, female mice were infertile. Luteal cell differentiation is impaired, and a disordered estrus cycle is detected. These results reflect a disturbance of the hypothalamic-pituitary-ovarian axis. The phenotypes of these mice suggest that loss of p27 causes an alteration in cell proliferation that can lead to specific endocrine dysfunction.",
author = "Hiroaki Kiyokawa and Kineman, {Rhonda D.} and Manova-Todorova, {Katia O.} and Soares, {Vera C.} and Huffman, {Eric S.} and Masao Ono and Dilruba Khanam and Hayday, {Adrian C.} and Frohman, {Lawrence A.} and Andrew Koff",
note = "Funding Information: Correspondence should be addressed to A. K. We thank Joan Massagu{\'e} and Kornelia Polyak for providing the p27 Kip1 cDNA prior to publication and David Morgan and Charles Sherr for baculoviruses encoding cyclins and CDKs. We are grateful for the helpful discussions and advice of Arch Perkins, Pier Paolo Pandolfi, Elizabeth Lacy, Lee Niswander, Roberta Rivi, Roger Pearse, Steve Swendeman, and our other colleagues at the institutes. We thank James Roberts and Kei-ichi Nakayama for communicating results prior to publication. We thank Birming Wong, Jia-Hui Dong, Karen Witty-Blease, Steve Bottega, Timothy J. Soos, Tom Taylor, and Michael Butz for excellent technical assistance. We thank Chris Fisher, Lee Niswander, and Joan Massagu{\'e}, for critical reading of the manuscript. This work was supported by grants from the National Institutes of Health to A. K. (GM52597), L. A. F. (NIDDK30667), and A. C. H. (GM37759), The Society of Memorial Sloan–Kettering Cancer Center to A. K., and The Bane Foundation to L. A. F. and a grant from the National Cancer Insititute to Memorial Sloan–Kettering Cancer Center. H. K. is supported by the DeWitt Wallace Research Fund of Memorial Sloan–Kettering Cancer Center.",
year = "1996",
month = may,
day = "31",
doi = "10.1016/S0092-8674(00)81238-6",
language = "English (US)",
volume = "85",
pages = "721--732",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "5",
}