Enhanced oligonucleotide binding to self-assembled nanofibers

Mustafa O. Guler; Jonathan K. Pokorski; Daniel H. Appella; Samuel I. Stupp

doi:10.1021/bc050053b

Enhanced oligonucleotide binding to self-assembled nanofibers

Mustafa O. Guler, Jonathan K. Pokorski, Daniel H. Appella, Samuel I. Stupp^*

^*Corresponding author for this work

Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A peptide nucleic acid/peptide amphiphile conjugate (PNA-PA) that self-assembles into fiber-shaped nanostructures was designed to bind oligonucleotides with high affinity and specificity. Oligonucleotide binding to PNA-PA nanofibers was studied using fluorescence polarization, and thermal stability was examined by UV-vis measurement of duplex melting temperatures. The self-assembled PNA-PA DNA system was observed to bind more strongly than the corresponding DNA-DNA duplex. We also observed single base specificity with a 16°C in thermal stability. As expected from the previous PNA studies, PNA-PA RNA binding is also stronger than the corresponding RNA-RNA duplex.

Original language	English (US)
Pages (from-to)	501-503
Number of pages	3
Journal	Bioconjugate Chemistry
Volume	16
Issue number	3
DOIs	https://doi.org/10.1021/bc050053b
State	Published - 2005

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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AU - Pokorski, Jonathan K.

AU - Appella, Daniel H.

AU - Stupp, Samuel I.

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AB - A peptide nucleic acid/peptide amphiphile conjugate (PNA-PA) that self-assembles into fiber-shaped nanostructures was designed to bind oligonucleotides with high affinity and specificity. Oligonucleotide binding to PNA-PA nanofibers was studied using fluorescence polarization, and thermal stability was examined by UV-vis measurement of duplex melting temperatures. The self-assembled PNA-PA DNA system was observed to bind more strongly than the corresponding DNA-DNA duplex. We also observed single base specificity with a 16°C in thermal stability. As expected from the previous PNA studies, PNA-PA RNA binding is also stronger than the corresponding RNA-RNA duplex.

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Enhanced oligonucleotide binding to self-assembled nanofibers

Abstract

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