Enhanced oligonucleotide binding to self-assembled nanofibers

Mustafa O. Guler, Jonathan K. Pokorski, Daniel H. Appella, Samuel I. Stupp*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


A peptide nucleic acid/peptide amphiphile conjugate (PNA-PA) that self-assembles into fiber-shaped nanostructures was designed to bind oligonucleotides with high affinity and specificity. Oligonucleotide binding to PNA-PA nanofibers was studied using fluorescence polarization, and thermal stability was examined by UV-vis measurement of duplex melting temperatures. The self-assembled PNA-PA DNA system was observed to bind more strongly than the corresponding DNA-DNA duplex. We also observed single base specificity with a 16°C in thermal stability. As expected from the previous PNA studies, PNA-PA RNA binding is also stronger than the corresponding RNA-RNA duplex.

Original languageEnglish (US)
Pages (from-to)501-503
Number of pages3
JournalBioconjugate Chemistry
Issue number3
StatePublished - 2005

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry


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