Enhanced recombination between λplac5 and F42lac: Identification of cis- and trans-acting factors

H. S. Seifert, R. D. Porter

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Enhanced transductional recombination between specialized transducing phase λplac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.

Original languageEnglish (US)
Pages (from-to)7500-7504
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number23 I
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • General

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