Enhanced susceptibility to Theiler's virus-induced demyelinating disease in perforin-deficient mice

Joann P. Palma; Hee Gu Lee; Mani Mohindru; Bong Su Kang; Mauro Dal Canto; Stephen D. Miller; Byung S. Kim

doi:10.1016/S0165-5728(01)00293-4

Enhanced susceptibility to Theiler's virus-induced demyelinating disease in perforin-deficient mice

Joann P. Palma, Hee Gu Lee, Mani Mohindru, Bong Su Kang, Mauro Dal Canto, Stephen D. Miller, Byung S. Kim^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Theiler's virus induces immune-mediated demyelinating disease similar to human MS in susceptible mice. Though the MHC class II-restricted T cell response is critical, susceptibility/resistance is also associated with a MHC class I haplotype. Here we report that perforin-deficient C57BL/6 mice (pKO) are susceptible to demyelination and develop clinical disease. The levels of primary demyelination, proliferation, Th1 responses, and viral load were also markedly enhanced. In addition, immunization of pKO mice with UV-inactivated virus further enhanced clinical incidence and accelerated the disease course. Thus, perforin is most likely involved in viral clearance, hence protection from the disease.

Original language	English (US)
Pages (from-to)	125-135
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	116
Issue number	2
DOIs	https://doi.org/10.1016/S0165-5728(01)00293-4
State	Published - Jun 1 2001

Funding

This work was supported by U.S. Public Health Service grants NS 28752, NS 33008, and NS 23349.

Keywords

CTL
Infectious immunity
Neuroimmunology
Virus

ASJC Scopus subject areas

Clinical Neurology
Neurology
Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0165-5728(01)00293-4

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title = "Enhanced susceptibility to Theiler's virus-induced demyelinating disease in perforin-deficient mice",

abstract = "Theiler's virus induces immune-mediated demyelinating disease similar to human MS in susceptible mice. Though the MHC class II-restricted T cell response is critical, susceptibility/resistance is also associated with a MHC class I haplotype. Here we report that perforin-deficient C57BL/6 mice (pKO) are susceptible to demyelination and develop clinical disease. The levels of primary demyelination, proliferation, Th1 responses, and viral load were also markedly enhanced. In addition, immunization of pKO mice with UV-inactivated virus further enhanced clinical incidence and accelerated the disease course. Thus, perforin is most likely involved in viral clearance, hence protection from the disease.",

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author = "Palma, {Joann P.} and Lee, {Hee Gu} and Mani Mohindru and Kang, {Bong Su} and {Dal Canto}, Mauro and Miller, {Stephen D.} and Kim, {Byung S.}",

note = "Funding Information: This work was supported by U.S. Public Health Service grants NS 28752, NS 33008, and NS 23349.",

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AU - Palma, Joann P.

AU - Lee, Hee Gu

AU - Mohindru, Mani

AU - Kang, Bong Su

AU - Dal Canto, Mauro

AU - Miller, Stephen D.

AU - Kim, Byung S.

N1 - Funding Information: This work was supported by U.S. Public Health Service grants NS 28752, NS 33008, and NS 23349.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Theiler's virus induces immune-mediated demyelinating disease similar to human MS in susceptible mice. Though the MHC class II-restricted T cell response is critical, susceptibility/resistance is also associated with a MHC class I haplotype. Here we report that perforin-deficient C57BL/6 mice (pKO) are susceptible to demyelination and develop clinical disease. The levels of primary demyelination, proliferation, Th1 responses, and viral load were also markedly enhanced. In addition, immunization of pKO mice with UV-inactivated virus further enhanced clinical incidence and accelerated the disease course. Thus, perforin is most likely involved in viral clearance, hence protection from the disease.

AB - Theiler's virus induces immune-mediated demyelinating disease similar to human MS in susceptible mice. Though the MHC class II-restricted T cell response is critical, susceptibility/resistance is also associated with a MHC class I haplotype. Here we report that perforin-deficient C57BL/6 mice (pKO) are susceptible to demyelination and develop clinical disease. The levels of primary demyelination, proliferation, Th1 responses, and viral load were also markedly enhanced. In addition, immunization of pKO mice with UV-inactivated virus further enhanced clinical incidence and accelerated the disease course. Thus, perforin is most likely involved in viral clearance, hence protection from the disease.

KW - CTL

KW - Infectious immunity

KW - Neuroimmunology

KW - Virus

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Enhanced susceptibility to Theiler's virus-induced demyelinating disease in perforin-deficient mice

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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