Enhancement of depolarization-dependent release of norepinephrine by an inhibitor of S-adenosylmethionine-dependent transmethylations

C. S. Rabe*, T. P. Williams, R. McGee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Depolarization-dependent release of [3H]-norepinephrine was studied using the pheochromocytoma clone, PC12. Norepinephrine release in response to nicotinic receptor stimulation or elevated K+ was enhanced substantially by 3-deazaadenosine, a compound known to cause inhibition of S-adenosylmethionine-dependent transmethylations. The enhancement was evident at a 3-deazaadenosine concentration as low as 10−5 M and increased with concentration. Simultaneous addition of L-homocysteine thiolactone increased the effects of 3-deazaadenosine. These results suggest that either transmethylations play some role in stimulation/secretion or that inhibition of transmethylations can indirectly enhance secretion.

Original languageEnglish (US)
Pages (from-to)1753-1759
Number of pages7
JournalLife Sciences
Volume27
Issue number19
DOIs
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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