Abstract
Depolarization-dependent release of [3H]-norepinephrine was studied using the pheochromocytoma clone, PC12. Norepinephrine release in response to nicotinic receptor stimulation or elevated K+ was enhanced substantially by 3-deazaadenosine, a compound known to cause inhibition of S-adenosylmethionine-dependent transmethylations. The enhancement was evident at a 3-deazaadenosine concentration as low as 10−5 M and increased with concentration. Simultaneous addition of L-homocysteine thiolactone increased the effects of 3-deazaadenosine. These results suggest that either transmethylations play some role in stimulation/secretion or that inhibition of transmethylations can indirectly enhance secretion.
Original language | English (US) |
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Pages (from-to) | 1753-1759 |
Number of pages | 7 |
Journal | Life Sciences |
Volume | 27 |
Issue number | 19 |
DOIs | |
State | Published - 1980 |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- Pharmacology, Toxicology and Pharmaceutics(all)