Abstract
Lipids are essential macromolecules that play a crucial role in numerous biological events. Lipids are structurally diverse which allows them to fulfill multiple functional roles. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a powerful tool to understand the spatial localization of lipids within biological systems. Herein, we report the use of ammonium fluoride (NH4F) as a comatrix additive to enhance lipid detection in biological samples, with a signal increase of up to 200%. Emphasis was placed on anionic lipid enhancement with negative polarity measurements, with some preliminary work on cationic lipids detailed. We observed lipid signal enhancement of [M-H]− ions with the addition of NH4F additive attributed to a proton transfer reaction in several different lipid classes. Overall, our study demonstrates that the use of the NH4F comatrix additive substantially improves sensitivity for lipid detection in a MALDI system and is capable of being applied to a variety of different applications.
Original language | English (US) |
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Pages (from-to) | 10603-10609 |
Number of pages | 7 |
Journal | Analytical Chemistry |
Volume | 95 |
Issue number | 28 |
DOIs | |
State | Published - Jul 18 2023 |
Funding
The authors acknowledge the financial support from the National Institutes of Health (NIH) (R01GM110406 and R21AG062144), NIH Director’s New Innovator Award (DP2CA271361), V Foundation Scholar Award, and startup funds from The Ohio State Comprehensive Cancer Center. Additionally, they would like to acknowledge that the graphical abstract was created with Biorender.com.
ASJC Scopus subject areas
- Analytical Chemistry