Enhancement of NIH3T3 cell proliferation by expressing macrophage colony stimulating factor in nuclei

Zhen Yu Cao, Ke Fu Wu*, Ge Li, Yong Min Lin, Bin Zhang, Guo Guang Zheng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Objective: To explore the effects of nuclear M-CSF on the process of tumorigenesis. Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei. The constructed plasmid was transferred into NIH3T3 cells and the cell clones were selected by G-418 selection. Cell clones stable expressing target protein were identified by RT-PCR, ABC immunohistochemistry assay and Western blot. Cell growth kinetics analyses through growth curves, cell doubling time, MTT test and anti-sense oligodeoxynucleotide (ASODN) inhibiting cell growth test were performed to identify cells proliferation potential. Results: The transfected cells showed elevated proliferation potential over the control cells. Conclusion: Abnormal appearance of M-CSF in nucleus could enhance cell proliferation, which suggests that cytokine isoforms within cell nucleus might play transcription factor-like role.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalChinese Journal of Cancer Research
Volume15
Issue number1
DOIs
StatePublished - Mar 2003

Keywords

  • Eukaryotic expression
  • Macrophage colony stimulating factor (M-CSF)
  • NIH3T3
  • Nuclear localization sequence (NLS)
  • Tumorigenesis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Enhancement of NIH3T3 cell proliferation by expressing macrophage colony stimulating factor in nuclei'. Together they form a unique fingerprint.

Cite this