Abstract
Objective: To explore the effects of nuclear M-CSF on the process of tumorigenesis. Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei. The constructed plasmid was transferred into NIH3T3 cells and the cell clones were selected by G-418 selection. Cell clones stable expressing target protein were identified by RT-PCR, ABC immunohistochemistry assay and Western blot. Cell growth kinetics analyses through growth curves, cell doubling time, MTT test and anti-sense oligodeoxynucleotide (ASODN) inhibiting cell growth test were performed to identify cells proliferation potential. Results: The transfected cells showed elevated proliferation potential over the control cells. Conclusion: Abnormal appearance of M-CSF in nucleus could enhance cell proliferation, which suggests that cytokine isoforms within cell nucleus might play transcription factor-like role.
Original language | English (US) |
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Pages (from-to) | 43-47 |
Number of pages | 5 |
Journal | Chinese Journal of Cancer Research |
Volume | 15 |
Issue number | 1 |
DOIs | |
State | Published - Mar 2003 |
Funding
Received date: November 18, 2002; Accepted date: January 17, 2003 Foundation item: This work was supported by a grant from Tianjin Science and Technology Development Project (No. 003119311). *Author to whom correspondence should be addressed. Phone: (0086-22)-27230400; Fax: (0086-22)-27306542; E-mail: kfwu @p ublic.tpt.tj.cn Biography: CAO Zhen-yu (1976-), male, graduated student, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, majors in cell biology.
Keywords
- Eukaryotic expression
- Macrophage colony stimulating factor (M-CSF)
- NIH3T3
- Nuclear localization sequence (NLS)
- Tumorigenesis
ASJC Scopus subject areas
- Oncology
- Cancer Research