TY - JOUR
T1 - Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis
AU - Hunter, Catherine J.
AU - Singamsetty, Vijay K.
AU - Chokshi, Nikunj K.
AU - Boyle, Patricia
AU - Camerini, Victoria
AU - Grishin, Anatoly V.
AU - Upperman, Jeffrey S.
AU - Ford, Henri R.
AU - Prasadarao, Nemani V.
N1 - Funding Information:
Received 19 June 2007; accepted 10 October 2007; electronically published 27 June 2008. Potential conflicts of interest: none reported. Presented in part: 27th Annual Meeting of the Surgical Infection Society, Toronto, 18–20 April 2007 (poster 4). Financial support: National Institute of Allergy and Infectious Diseases, National Institutes of Health (grants AI 40567 to N.V.P. and AI 49473 to H.R.F.); Surgical Infection Society (research fellowship award to C.J.H.). Reprints or correspondence: Dr. Nemani Prasadarao, Div. of Infectious Diseases, MS 51, Childrens Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027 (pnemani@chla.usc.edu).
PY - 2008/8/15
Y1 - 2008/8/15
N2 - Necrotizing enterocolitis (NEC) is an inflammatory intestinal disorder that affects 2%-5% of all premature infants. Enterobacter sakazakii, a common contaminant of milk-based powdered infant formula, has been implicated as a causative agent of sepsis, meningitis, and NEC in newborn infants, with high mortality rates. However, the role played by E. sakazakii in the pathogenesis of NEC is, to date, not known. Here, we demonstrate for the first time that E. sakazakii can induce clinical and histological NEC in newborn rats. E. sakazakii was found to bind to enterocytes in rat pups at the tips of villi and to intestinal epithelial cells (IEC-6) in culture, with no significant invasion. Exposure to E. sakazakii induced apoptosis and increased the production of interleukin-6 in IEC-6 cells and in the animal model. These data suggest that E. sakazakii could be a potential pathogen that induces NEC and triggers intestinal disease by modulating enterocyte intracellular signaling pathways.
AB - Necrotizing enterocolitis (NEC) is an inflammatory intestinal disorder that affects 2%-5% of all premature infants. Enterobacter sakazakii, a common contaminant of milk-based powdered infant formula, has been implicated as a causative agent of sepsis, meningitis, and NEC in newborn infants, with high mortality rates. However, the role played by E. sakazakii in the pathogenesis of NEC is, to date, not known. Here, we demonstrate for the first time that E. sakazakii can induce clinical and histological NEC in newborn rats. E. sakazakii was found to bind to enterocytes in rat pups at the tips of villi and to intestinal epithelial cells (IEC-6) in culture, with no significant invasion. Exposure to E. sakazakii induced apoptosis and increased the production of interleukin-6 in IEC-6 cells and in the animal model. These data suggest that E. sakazakii could be a potential pathogen that induces NEC and triggers intestinal disease by modulating enterocyte intracellular signaling pathways.
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U2 - 10.1086/590186
DO - 10.1086/590186
M3 - Article
C2 - 18588483
AN - SCOPUS:48749129994
SN - 0022-1899
VL - 198
SP - 586
EP - 593
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
