Abstract
Rats were kindled to two consecutive class 5 seizures by once-daily entorhinal electrical stimulation. After one stimulus-free month, in vitro Ca2+-dependent, K+-stimulated endogenous amino acid release was measured in regio superior, regio inferior and dentate gyrus of the hippocampal formation. Ca2+-dependent l-glutamate release was robust in all 3 regions of controls and greatest in dentate gyrus; release of GABA and l-aspartate were significant in regio superior and dentate gyrus. l-Glutamate release was significantly enhanced in ipsilateral regio inferior of kindled hippocampus and tended to be greater contralaterally. This pattern was not seen in regio superior or dentate gyrus. These studies, in concert with others, suggest that Ca2+-dependent l-glutamate release in hippocampus is augmented by entorhinal kindling and that this enhanced release may be primarily from presynaptic granule cell mossy fiber projections.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 188-193 |
| Number of pages | 6 |
| Journal | Brain research |
| Volume | 508 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 5 1990 |
Funding
Acknowledgements. The authors thank Ramona M. Alcala and Lindley P. Wemmer for their expert technical assistance and Renee Elkins for typing the manuscript. This research was supported by the Veterans Administration Research Service and the Bertha LeBus Educational and Charitable Trust.
Keywords
- Ca
- Excitatory amino acid
- Glutamate
- Hippocampus
- Kindling
- Long-term potentiation
- Neurotransmitter release
- Regio inferior
ASJC Scopus subject areas
- Clinical Neurology
- Molecular Biology
- General Neuroscience
- Developmental Biology