Entrapment of a highly specific antiprogesterone antiserum using polysiloxane copolymers

Duane L. Venton; Kerry L. Cheesman; Robert T. Chatterton; Thaddeus L. Anderson

doi:10.1016/0304-4165(84)90255-1

Entrapment of a highly specific antiprogesterone antiserum using polysiloxane copolymers

Duane L. Venton^*, Kerry L. Cheesman, Robert T. Chatterton, Thaddeus L. Anderson

^*Corresponding author for this work

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Antiprogesterone antiserum was entrapped within a polysiloxane copolymer prepared from a 3:1 mixture of tetraethoxysilane and 3-aminopropyltriethoxysilane monomers. 400 μl of thhis monomer mixture entrapped 70 mg of the 140 mg of immunoglobulins which were added, and the protein could not be washed from the highly stable copolymer which formed. Approximately half of the entrapped antiprogesterone antiserum was found to retain progesterone binding capacity with an apparent K_a equal to that of free antiserum in solution and was insensitive to effects of pH between 3 and 7. These preliminary observations and the unique chemistry of polysiloxane polymer formation suggest that such polymers may be useful in the entrapment of proteins for a variety of applications.

Original language	English (US)
Pages (from-to)	343-347
Number of pages	5
Journal	BBA - General Subjects
Volume	797
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(84)90255-1
State	Published - Mar 1 1984

Funding

This work was supportedin part by subcontract 203 from the Program for Applied Researchi n Fertility Regulation.

Keywords

Polysiloxane copolymer
Protein entrapment

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

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10.1016/0304-4165(84)90255-1

Cite this

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title = "Entrapment of a highly specific antiprogesterone antiserum using polysiloxane copolymers",

abstract = "Antiprogesterone antiserum was entrapped within a polysiloxane copolymer prepared from a 3:1 mixture of tetraethoxysilane and 3-aminopropyltriethoxysilane monomers. 400 μl of thhis monomer mixture entrapped 70 mg of the 140 mg of immunoglobulins which were added, and the protein could not be washed from the highly stable copolymer which formed. Approximately half of the entrapped antiprogesterone antiserum was found to retain progesterone binding capacity with an apparent Ka equal to that of free antiserum in solution and was insensitive to effects of pH between 3 and 7. These preliminary observations and the unique chemistry of polysiloxane polymer formation suggest that such polymers may be useful in the entrapment of proteins for a variety of applications.",

keywords = "Polysiloxane copolymer, Protein entrapment",

author = "Venton, \{Duane L.\} and Cheesman, \{Kerry L.\} and Chatterton, \{Robert T.\} and Anderson, \{Thaddeus L.\}",

note = "Funding Information: This work was supportedin part by subcontract 203 from the Program for Applied Researchi n Fertility Regulation.",

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AU - Venton, Duane L.

AU - Cheesman, Kerry L.

AU - Chatterton, Robert T.

AU - Anderson, Thaddeus L.

N1 - Funding Information: This work was supportedin part by subcontract 203 from the Program for Applied Researchi n Fertility Regulation.

PY - 1984/3/1

Y1 - 1984/3/1

N2 - Antiprogesterone antiserum was entrapped within a polysiloxane copolymer prepared from a 3:1 mixture of tetraethoxysilane and 3-aminopropyltriethoxysilane monomers. 400 μl of thhis monomer mixture entrapped 70 mg of the 140 mg of immunoglobulins which were added, and the protein could not be washed from the highly stable copolymer which formed. Approximately half of the entrapped antiprogesterone antiserum was found to retain progesterone binding capacity with an apparent Ka equal to that of free antiserum in solution and was insensitive to effects of pH between 3 and 7. These preliminary observations and the unique chemistry of polysiloxane polymer formation suggest that such polymers may be useful in the entrapment of proteins for a variety of applications.

AB - Antiprogesterone antiserum was entrapped within a polysiloxane copolymer prepared from a 3:1 mixture of tetraethoxysilane and 3-aminopropyltriethoxysilane monomers. 400 μl of thhis monomer mixture entrapped 70 mg of the 140 mg of immunoglobulins which were added, and the protein could not be washed from the highly stable copolymer which formed. Approximately half of the entrapped antiprogesterone antiserum was found to retain progesterone binding capacity with an apparent Ka equal to that of free antiserum in solution and was insensitive to effects of pH between 3 and 7. These preliminary observations and the unique chemistry of polysiloxane polymer formation suggest that such polymers may be useful in the entrapment of proteins for a variety of applications.

KW - Polysiloxane copolymer

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Entrapment of a highly specific antiprogesterone antiserum using polysiloxane copolymers

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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