Enzymes have been proposed as tissue receptors that bind 99Tc(m) stannous diphosphonate and its analogs. Incubation of diphosphonate with several enzymes demonstrated inhibition of acid and alkaline phosphatase activity but showed no effect on glutamic oxalacetic transaminase and lactate dehydrogenase activity. Complete reversal of the diphosphonate induced inhibition of alkaline phosphatase activity occurred when calcium ion was added to the reaction. The specificity of calcium to induce reversal was dispelled when magnesium ion gave identical results. Diphosphonate induced inhibition of acid phosphatase, however, was not reversed by calcium or magnesium.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Nuclear Medicine|
|State||Published - Dec 1 1975|
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging