Enzymatic Oligoribonucleotide Synthesis with T4 RNA Ligase

Thomas E. England, Olke C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticle

181 Scopus citations

Abstract

The substrate specificity of T4 RNA ligase has been examined to determine whether the intermolecular reaction is sufficiently general to realize its potential in the enzymatic synthesis of oligoribonucleotides of defined sequence. Reactions between a variety of acceptor molecules with 3′- and 5′-hydroxyl groups and donor molecules with 3′- and 5′-phosphates indicate that the minimal substrates are a trinucleoside diphosphate acceptor and a nucleoside 3′5′-bisphosphate donor. Increasing the chain length of either the acceptor or donor has little effect on the rate or extent of reaction. Although the base composition of the donor has only a small effect on the reaction rate, the presence of uridine in the acceptor greatly reduces the amount of product formed. The presence of a phosphate on the 3′ terminus of the donor molecule permits a unique intermolecular product with a 5′-hydroxyl and a 3′-phosphate. By enzymatically either adding a 5′-phosphate or removing the 3′-phosphate, a new donor or acceptor is prepared so synthesis of an oligomer chain can proceed in either direction. With the simplicity of this enzymatic pathway and the rather broad substrate specificity of T4 RNA ligase, a convenient method for the synthesis of oligoribonucleotides is established.

Original languageEnglish (US)
Pages (from-to)2069-2076
Number of pages8
JournalBiochemistry
Volume17
Issue number11
DOIs
StatePublished - Jan 1 1978

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Enzymatic Oligoribonucleotide Synthesis with T4 RNA Ligase'. Together they form a unique fingerprint.

  • Cite this