TY - JOUR
T1 - Enzymatic properties of the phosphorylated urokinase-type plasminogen activator isolated from a human carcinomatous cell line
AU - Takahashi, Kei
AU - Kwaan, Hau C.
AU - Koh, Enki
AU - Tanabe, Masataka
N1 - Funding Information:
This research is supported in part by a Grant-in-Aid (63570038) for Scientific Research fran the Ministry of Education, Science, and Culture, Japan.
PY - 1992/2/14
Y1 - 1992/2/14
N2 - Enzymatic properties of phosphorylated urokinase plasminogen activator (P-uPA) (1) extracted from human carcinomatous cell line Detroit 562 cells were compared with those of non-phosphorylated uPA of urinary origin (nP-uPA). Using plasminogen as a subsrate, the Km and Kcat of P-uPA were higher than that of nP-uPA while the Kcat Km was lower. By zymography, a greater degree of plasminogen activation was observed. Concanavalin A reacted to both the enzymes. P-uPA had a low affinity for the inhibitors of plasminogen activator PAI-1 and PAI-2, and was inhibited only by the excess amounts of inhibitors. For PAI-1, the KIs of P-uPA was greater and for PAI-2, KI was higher for P-uPA. These alterations by phosphorylation enable uPA to be more efficient in a focal proteolysis through plasminogen activation.
AB - Enzymatic properties of phosphorylated urokinase plasminogen activator (P-uPA) (1) extracted from human carcinomatous cell line Detroit 562 cells were compared with those of non-phosphorylated uPA of urinary origin (nP-uPA). Using plasminogen as a subsrate, the Km and Kcat of P-uPA were higher than that of nP-uPA while the Kcat Km was lower. By zymography, a greater degree of plasminogen activation was observed. Concanavalin A reacted to both the enzymes. P-uPA had a low affinity for the inhibitors of plasminogen activator PAI-1 and PAI-2, and was inhibited only by the excess amounts of inhibitors. For PAI-1, the KIs of P-uPA was greater and for PAI-2, KI was higher for P-uPA. These alterations by phosphorylation enable uPA to be more efficient in a focal proteolysis through plasminogen activation.
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U2 - 10.1016/0006-291X(92)91900-B
DO - 10.1016/0006-291X(92)91900-B
M3 - Article
C2 - 1540190
AN - SCOPUS:0026596499
SN - 0006-291X
VL - 182
SP - 1473
EP - 1481
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -