Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai, Lan Ban, Wanyi Guan, Milan Mrksich, Peng George Wang*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

Original languageEnglish (US)
Pages (from-to)1576-1580
Number of pages5
JournalCarbohydrate Research
Volume346
Issue number12
DOIs
StatePublished - Sep 6 2011

Keywords

  • Enzymatic synthesis
  • Glycosylation
  • SAMDI MS
  • UDP-GlcNAc

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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