Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai; Lan Ban; Wanyi Guan; Milan Mrksich; Peng George Wang

doi:10.1016/j.carres.2011.05.005

Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai, Lan Ban, Wanyi Guan, Milan Mrksich, Peng George Wang^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

Original language	English (US)
Pages (from-to)	1576-1580
Number of pages	5
Journal	Carbohydrate Research
Volume	346
Issue number	12
DOIs	https://doi.org/10.1016/j.carres.2011.05.005
State	Published - Sep 6 2011

Keywords

Enzymatic synthesis
Glycosylation
SAMDI MS
UDP-GlcNAc

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.carres.2011.05.005

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title = "Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine",

abstract = "This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.",

keywords = "Enzymatic synthesis, Glycosylation, SAMDI MS, UDP-GlcNAc",

author = "Li Cai and Lan Ban and Wanyi Guan and Milan Mrksich and Wang, {Peng George}",

note = "Funding Information: P.G.W. acknowledges NIH ( R01 AI083754 , R01 HD061935 and R01 GM085267 ) for financial support. ",

year = "2011",

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doi = "10.1016/j.carres.2011.05.005",

language = "English (US)",

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T1 - Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

AU - Cai, Li

AU - Ban, Lan

AU - Guan, Wanyi

AU - Mrksich, Milan

AU - Wang, Peng George

N1 - Funding Information: P.G.W. acknowledges NIH ( R01 AI083754 , R01 HD061935 and R01 GM085267 ) for financial support.

PY - 2011/9/6

Y1 - 2011/9/6

N2 - This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

AB - This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

KW - Enzymatic synthesis

KW - Glycosylation

KW - SAMDI MS

KW - UDP-GlcNAc

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DO - 10.1016/j.carres.2011.05.005

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JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

IS - 12

ER -

Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Abstract

Keywords

ASJC Scopus subject areas

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