Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai; Lan Ban; Wanyi Guan; Milan Mrksich; Peng George Wang

doi:10.1016/j.carres.2011.05.005

Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai, Lan Ban, Wanyi Guan, Milan Mrksich, Peng George Wang^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article

8 Scopus citations

Abstract

This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

Original language	English (US)
Pages (from-to)	1576-1580
Number of pages	5
Journal	Carbohydrate Research
Volume	346
Issue number	12
DOIs	https://doi.org/10.1016/j.carres.2011.05.005
State	Published - Sep 6 2011

Keywords

Enzymatic synthesis
Glycosylation
SAMDI MS
UDP-GlcNAc

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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abstract = "This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.",

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AU - Ban, Lan

AU - Guan, Wanyi

AU - Mrksich, Milan

AU - Wang, Peng George

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KW - Glycosylation

KW - SAMDI MS

KW - UDP-GlcNAc

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