Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai; Lan Ban; Wanyi Guan; Milan Mrksich; Peng George Wang

doi:10.1016/j.carres.2011.05.005

Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Li Cai, Lan Ban, Wanyi Guan, Milan Mrksich, Peng George Wang^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

Original language	English (US)
Pages (from-to)	1576-1580
Number of pages	5
Journal	Carbohydrate Research
Volume	346
Issue number	12
DOIs	https://doi.org/10.1016/j.carres.2011.05.005
State	Published - Sep 6 2011

Funding

P.G.W. acknowledges NIH ( R01 AI083754 , R01 HD061935 and R01 GM085267 ) for financial support.

Keywords

Enzymatic synthesis
Glycosylation
SAMDI MS
UDP-GlcNAc

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

Access to Document

10.1016/j.carres.2011.05.005

Cite this

@article{1827a1d2072443239608f1d0cd6d5e87,

title = "Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine",

abstract = "This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.",

keywords = "Enzymatic synthesis, Glycosylation, SAMDI MS, UDP-GlcNAc",

author = "Li Cai and Lan Ban and Wanyi Guan and Milan Mrksich and Wang, {Peng George}",

note = "Funding Information: P.G.W. acknowledges NIH ( R01 AI083754 , R01 HD061935 and R01 GM085267 ) for financial support. ",

year = "2011",

month = sep,

day = "6",

doi = "10.1016/j.carres.2011.05.005",

language = "English (US)",

volume = "346",

pages = "1576--1580",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd",

number = "12",

}

TY - JOUR

T1 - Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

AU - Cai, Li

AU - Ban, Lan

AU - Guan, Wanyi

AU - Mrksich, Milan

AU - Wang, Peng George

N1 - Funding Information: P.G.W. acknowledges NIH ( R01 AI083754 , R01 HD061935 and R01 GM085267 ) for financial support.

PY - 2011/9/6

Y1 - 2011/9/6

N2 - This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

AB - This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.

KW - Enzymatic synthesis

KW - Glycosylation

KW - SAMDI MS

KW - UDP-GlcNAc

UR - http://www.scopus.com/inward/record.url?scp=79959931948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959931948&partnerID=8YFLogxK

U2 - 10.1016/j.carres.2011.05.005

DO - 10.1016/j.carres.2011.05.005

M3 - Article

C2 - 21645883

AN - SCOPUS:79959931948

SN - 0008-6215

VL - 346

SP - 1576

EP - 1580

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 12

ER -

Enzymatic synthesis and properties of uridine-5′-O-(2-thiodiphospho)- N-acetylglucosamine

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this