Abstract
This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.
Original language | English (US) |
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Pages (from-to) | 1576-1580 |
Number of pages | 5 |
Journal | Carbohydrate Research |
Volume | 346 |
Issue number | 12 |
DOIs | |
State | Published - Sep 6 2011 |
Funding
P.G.W. acknowledges NIH ( R01 AI083754 , R01 HD061935 and R01 GM085267 ) for financial support.
Keywords
- Enzymatic synthesis
- Glycosylation
- SAMDI MS
- UDP-GlcNAc
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry