Enzyme immunoassay for total immunoglobulin E in dried blood spots

Susan Tanner*, Thomas McDade

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Elevated circulating levels of total immunoglobulin E (IgE) are associated with both allergic disease and repeated macro-parasitic infections. Population-based research on IgE has been limited by the logistical constraints associated with obtaining and processing venipuncture blood samples. In this short report, we present an enzyme immunoassay protocol for quantifying circulating total IgE levels in capillary whole blood, collected from a finger prick and dried on filter paper. The assay demonstrated acceptable levels of accuracy, precision, and reliability. IgE remained stable at room temperature for only 2-4 days and degraded rapidly at higher temperatures suggesting that samples should be refrigerated or frozen within 1-2 days of collection. It is hoped that the relative ease of blood spot collection will expand opportunities for population-based research on IgE.

Original languageEnglish (US)
Pages (from-to)440-442
Number of pages3
JournalAmerican Journal of Human Biology
Volume19
Issue number3
DOIs
StatePublished - May 1 2007

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Anthropology

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