Eosinophil granule-derived major basic protein induces IL-8 expression in human intestinal myofibroblasts

G. T. Furuta*, S. J. Ackerman, J. Varga, A. M. Spiess, M. Y. Wang, B. K. Wershil

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Eosinophil infiltration occurs in a variety of allergic and inflammatory diseases. The release of preformed mediators from eosinophils may contribute to inflammatory responses. We investigated the ability of eosinophil-derived major basic protein and eosinophil-derived neurotoxin to stimulate production of IL-8 from intestinal myofibroblasts. Intestinal myofibroblasts (18-Co cells) were incubated with major basic protein, eosinophil-derived neurotoxin, or a synthetic analogue of major basic protein, poly-L-arginine. Immunoreactive IL-8 was measured by ELISA and IL-8 mRNA levels were analysed by Northern blot or reverse transcription-polymerase chain assay. Major basic protein induced IL-8 mRNA production and release of significant levels of IL-8 immunoreactive protein. By contrast, eosinophil-derived neurotoxin stimulated little IL-8 release. The induction of IL-8 mRNA by poly-L-arginine was significantly inhibited by actinomycin D. These findings demonstrate a novel interaction between eosinophils and intestinal fibroblasts that may be involved in the pathogenesis of diseases associated with tissue eosinophilia.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalClinical and Experimental Immunology
Volume122
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Eosinophil
  • Fibroblast
  • Gastrointestinal
  • IL-8
  • Major basic protein

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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