TY - JOUR
T1 - Ependymomas in children express the multidrug resistance gene
T2 - Immunohistochemical and molecular biologic study
AU - Chou, Pauline M.
AU - Barquin, Nora
AU - Gonzalez-Crussi, Frank
AU - Sanz, Cecilia Ridaura
AU - Tomita, Tadanori
AU - Reyes-Mugica, Miguel
N1 - Funding Information:
Received IOJuly 1995; accepted 5 October 1995. Supported in part by The Children’s Memorial Institute for Education and Research Starter Grant. Parts of’ this work were presented at the Annual International Academy of Pathology meeting, San Francisco, March 1994. The authors would like to express their thanks to Drs. Roninson and Metchetner for their generosity in providing us with UIC2 antibody, and to Drs. X. Tan, M.M. Myint, and Takasumi Yasuda for their technical assistance. Address correspondence to Pauline M. Chou, MD, Department of Pathology, Box 17, Children’s Memorial Hospital, Chicago, IL 60614, USA.
PY - 1996
Y1 - 1996
N2 - In the view of the poor response of ependymomas to chemotherapy, it may be hypothesized that these tumors have intrinsic drug resistance to some chemotherapeutic agents. The expression of drug resistance may be specific to a single agent or may involve multiple drugs. Among several mechanisms of drug resistance. P-glycoprotein (Pgp) has been the subject of considerable attention in clinical practice. In order to assess the possible participation of Pgp in the chemotherapeutic resistance of ependymomas, 42 biopsy specimens from 35 patients with ependymoma seen at our institutions were studied by immonohistochemistry with two monoclonal antibodies: C219 (Signet) and UIC-2 (Dr. Roninson's gift). In addition, four cases were by polymerase chain reaction after reverse transcription to detect transcripts of Pgp. Our results showed than in 35 samples there was a positive reaction for Pgp with both antibodies; two biopsy samples were positive only with C219 and three others with UIC-2; the remaining two samples were negative with both antibodies. Of the four cases studied by RT-PCR, three showed MDR1 transcripts. These results support our hypothesis of Pgp-mediated intrinsic multidrug resistance in these tumors.
AB - In the view of the poor response of ependymomas to chemotherapy, it may be hypothesized that these tumors have intrinsic drug resistance to some chemotherapeutic agents. The expression of drug resistance may be specific to a single agent or may involve multiple drugs. Among several mechanisms of drug resistance. P-glycoprotein (Pgp) has been the subject of considerable attention in clinical practice. In order to assess the possible participation of Pgp in the chemotherapeutic resistance of ependymomas, 42 biopsy specimens from 35 patients with ependymoma seen at our institutions were studied by immonohistochemistry with two monoclonal antibodies: C219 (Signet) and UIC-2 (Dr. Roninson's gift). In addition, four cases were by polymerase chain reaction after reverse transcription to detect transcripts of Pgp. Our results showed than in 35 samples there was a positive reaction for Pgp with both antibodies; two biopsy samples were positive only with C219 and three others with UIC-2; the remaining two samples were negative with both antibodies. Of the four cases studied by RT-PCR, three showed MDR1 transcripts. These results support our hypothesis of Pgp-mediated intrinsic multidrug resistance in these tumors.
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U2 - 10.1080/15513819609168692
DO - 10.1080/15513819609168692
M3 - Article
C2 - 9025853
AN - SCOPUS:0029766571
SN - 1551-3815
VL - 16
SP - 551
EP - 561
JO - Pediatric Pathology and Molecular Medicine
JF - Pediatric Pathology and Molecular Medicine
IS - 4
ER -