Ephrin-As guide the formation of functional maps in the visual cortex

Jianhua Cang; Megumi Kaneko; Jena Yamada; Georgia Woods; Michael P. Stryker; David A. Feldheim

doi:10.1016/j.neuron.2005.10.026

Ephrin-As guide the formation of functional maps in the visual cortex

Jianhua Cang, Megumi Kaneko, Jena Yamada, Georgia Woods, Michael P. Stryker^*, David A. Feldheim

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

148 Scopus citations

Abstract

Ephrin-As and their receptors, EphAs, are expressed in the developing cortex where they may act to organize thalamic inputs. Here, we map the visual cortex (V1) in mice deficient for ephrin-A2, -A3, and -A5 functionally, using intrinsic signal optical imaging and microelectrode recording, and structurally, by anatomical tracing of thalamocortical projections. V1 is shifted medially, rotated, and compressed and its internal organization is degraded. Expressing ephrin-A5 ectopically by in utero electroporation in the lateral cortex shifts the map of V1 medially, and expression within V1 disrupts its internal organization. These findings indicate that interactions between gradients of EphA/ephrin-A in the cortex guide map formation, but that factors other than redundant ephrin-As are responsible for the remnant map. Together with earlier work on the retinogeniculate map, the current findings show that the same molecular interactions may operate at successive stages of the visual pathway to organize maps.

Original language	English (US)
Pages (from-to)	577-589
Number of pages	13
Journal	Neuron
Volume	48
Issue number	4
DOIs	https://doi.org/10.1016/j.neuron.2005.10.026
State	Published - Nov 23 2005

Funding

The work was supported by U.S. National Institutes of Health (N.I.H.) grants EY014689 (D.A.F.) and EY02874 (M.P.S.). J.C. is an Aventis Pharmaceuticals Fellow of the Life Sciences Research Foundation. We thank Ann Schreiber, for help with brain sectioning; Hema Vaidyanathan, Cory Pfeiffenberger, Ben Stafford, and Pierre Vanderhaeghen, for critical reading of the manuscript; and Andrew Huberman and members of the Feldheim and Stryker labs, for thoughtful discussion.

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2005.10.026

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title = "Ephrin-As guide the formation of functional maps in the visual cortex",

abstract = "Ephrin-As and their receptors, EphAs, are expressed in the developing cortex where they may act to organize thalamic inputs. Here, we map the visual cortex (V1) in mice deficient for ephrin-A2, -A3, and -A5 functionally, using intrinsic signal optical imaging and microelectrode recording, and structurally, by anatomical tracing of thalamocortical projections. V1 is shifted medially, rotated, and compressed and its internal organization is degraded. Expressing ephrin-A5 ectopically by in utero electroporation in the lateral cortex shifts the map of V1 medially, and expression within V1 disrupts its internal organization. These findings indicate that interactions between gradients of EphA/ephrin-A in the cortex guide map formation, but that factors other than redundant ephrin-As are responsible for the remnant map. Together with earlier work on the retinogeniculate map, the current findings show that the same molecular interactions may operate at successive stages of the visual pathway to organize maps.",

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note = "Funding Information: The work was supported by U.S. National Institutes of Health (N.I.H.) grants EY014689 (D.A.F.) and EY02874 (M.P.S.). J.C. is an Aventis Pharmaceuticals Fellow of the Life Sciences Research Foundation. We thank Ann Schreiber, for help with brain sectioning; Hema Vaidyanathan, Cory Pfeiffenberger, Ben Stafford, and Pierre Vanderhaeghen, for critical reading of the manuscript; and Andrew Huberman and members of the Feldheim and Stryker labs, for thoughtful discussion. ",

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N2 - Ephrin-As and their receptors, EphAs, are expressed in the developing cortex where they may act to organize thalamic inputs. Here, we map the visual cortex (V1) in mice deficient for ephrin-A2, -A3, and -A5 functionally, using intrinsic signal optical imaging and microelectrode recording, and structurally, by anatomical tracing of thalamocortical projections. V1 is shifted medially, rotated, and compressed and its internal organization is degraded. Expressing ephrin-A5 ectopically by in utero electroporation in the lateral cortex shifts the map of V1 medially, and expression within V1 disrupts its internal organization. These findings indicate that interactions between gradients of EphA/ephrin-A in the cortex guide map formation, but that factors other than redundant ephrin-As are responsible for the remnant map. Together with earlier work on the retinogeniculate map, the current findings show that the same molecular interactions may operate at successive stages of the visual pathway to organize maps.

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Ephrin-As guide the formation of functional maps in the visual cortex

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