Epithelial genes in chronic rhinosinusitis with and without nasal polyps

Sara L. Richer, Ai Q. Truong-Tran, David B. Conley, Roderick Carter, David Vermylen, Leslie C. Grammer, Anju T. Peters, Rakesh K. Chandra, Kathleen E. Harris, Robert C. Kern, Robert P. Schleimer

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Background: Genetic studies on chronic inflammatory diseases have resulted in an emphasis on the epithelial interface with the environment and the genes that influence this interaction. This study examines the expression of key epithelial genes implicated in the pathogenesis of other inflammatory disorders for their role in chronic rhinosinusitis (CRS). Methods: Epithelial cells were collected from the inferior turbinate, middle turbinate, and/or uncinate from 62 subjects undergoing sinonasal surgery. Patient groups included 21 CRS patients with nasal polyposis, 23 CRS patients without nasal polyposis, and 18 controls. Samples were analyzed for S100A7, S100A8, S100A9, SLC9A3R1, G-protein-coupled receptor for asthma, and serine protease inhibitor kazal type 5 (SPINK5) by quantitative real-time polymerase chain reaction. Immunohistochemistry (IHC) was performed to analyze expression of SPINK5 lympho epithelial kazal-type inhibitor (LEKTI) in sinonasal samples. Results: Expression of S100A7 and S100A8 was significantly decreased in CRS with and without nasal polyps when compared with controls. S100A9 expression was significantly decreased in CRS without nasal polyps, and SPINKS expression was significantly decreased in CRS with nasal polyps. SPINK5 (LEKTI) protein was detected in sinonasal tissue and was significantly decreased in polyp samples using IHC. Conclusion: This study shows marked reductions in the level of expression of several genes involved in epithelial barrier maintenance and repair in the inflammatory state of CRS.

Original languageEnglish (US)
Pages (from-to)228-234
Number of pages7
JournalAmerican Journal of Rhinology
Volume22
Issue number3
DOIs
StatePublished - May 2008

Keywords

  • Chronic rhinosinusitis
  • Epithelial genes
  • Inflammation
  • LEKTI
  • Nasal polyps
  • S100A7
  • S100A8
  • S100A9
  • SPINK5

ASJC Scopus subject areas

  • Otorhinolaryngology

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