Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence

J. L. Millan, C. E. Driscoll, K. M. LeVan, E. Goldberg

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

The sequence and structure of human testis-specific L-lactate dehydrogenase [LDHC4, LDHX; (L)-lactate:NAD+ oxidoreductase, EC 1.1.1.27] has been derived from analysis of a complementary DNA (cDNA) clone comprising the complete protein coding region of the enzyme. From the deduced amino acid sequence, human LDHC4 is as different from rodent LDHC4 (73% homology) as it is from human LDHA4 (76% homology) and porcine LDHB4 (68% homology). Subunit homologies are consistent with the conclusion that the LDHC gene arose by at least two independent duplication events. Furthermore, the lower degree of homology between mouse and human LDHC4 and the appearance of this isozyme late in evolution suggests a higher rate of mutation in the mammalian LDHC genes than in the LDHA and -B genes. Comparison of exposed amino acid residues of discrete antigenic determinants of mouse and human LDHC4 reveals significant differences. Knowledge of the human LDHC4 sequence will help design human-specific peptides useful in the development of a contraceptive vaccine.

Original languageEnglish (US)
Pages (from-to)5311-5315
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number15
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence'. Together they form a unique fingerprint.

Cite this