EPR and ENDOR Detection of Compound I from Micrococcus lysodeikticus Catalase

Michael J. Benecky*, Brian M. Hoffman, Jane E. Frew, Nina Scowen, Peter Jones

*Corresponding author for this work

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

We present the first EPR and ENDOR examination of a catalase compound I (Cat I), the one formed by peracetic acid treatment of Micrococcus lysodeikticus catalase. The Cat I rapid-passage EPR signal (geff = 3.32; geff ∼ 2) appears quite different from those reported previously for the compounds I from horseradish peroxidase (HRP I) and chloroperoxidase. Nonetheless, all three signals can be explained by the same model for exchange coupling between an S = 1 oxoferryl [Fe=O]2+ moiety and a porphyrin π-cation radical (S′ = 1/2) (Schulz, C. E., et al. (1979) FEBS Lett. 103, 102–105). The signal for Cat I is unlike those for the two peroxidases in that it reflects a ferromagnetic rather than antiferromagnetic exchange. Preliminary 1H ENDOR spectra for Cat I appear to differ from the proton (1H) ENDOR spectra of HRP I; the latter, along with the 14N ENDOR spectra, indicate that the porphyrin radical in HRP I exhibits a predominantly A2u-like state having large spin densities on porphyrin N and C(β). The proton ENDOR spectrum of Cat I is insensitive to H/D exchange, which indicates that the [Fe=O]2+ moiety is not protonated. Consideration of the EPR results for a series of compounds I suggests that the sign and magnitude of the exchange parameter (J) is correlated with the nature of the proximal axial ligand.

Original languageEnglish (US)
Pages (from-to)11929-11933
Number of pages5
JournalBiochemistry
Volume32
Issue number44
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry

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