Epstein-Barr virus LMP2A drives B cell development and survival in the absence of normal B cell receptor signals

Robert G. Caldwell; Joanna B. Wilson; Steven J. Anderson; Richard Longnecker

doi:10.1016/S1074-7613(00)80623-8

Epstein-Barr virus LMP2A drives B cell development and survival in the absence of normal B cell receptor signals

Robert G. Caldwell, Joanna B. Wilson, Steven J. Anderson, Richard Longnecker^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

483 Scopus citations

Abstract

Epstein-Barr virus (EBV) establishes a persistent latent infection in peripheral B lymphocytes in humans and is associated with a variety of malignancies and proliferative disorders. Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed in latently infected B lymphocytes in vivo. LMP2A blocks B cell receptor (BCR) signal transduction in vitro by binding the Syk and Lyn protein tyrosine kinases. To analyze the significance of LMP2A expression in vivo, transgenic mice with B cell lineage expression of LMP2A were generated. LMP2A expression results in the bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin- negative cells to colonize peripheral lymphoid organs, indicating that LMP2A possesses a constitutive signaling activity in nontransformed cells.

Original language	English (US)
Pages (from-to)	405-411
Number of pages	7
Journal	Immunity
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/S1074-7613(00)80623-8
State	Published - Sep 1998

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S1074-7613(00)80623-8

Cite this

@article{b43bfb286d3b42989b8bbdc06fc7e488,

title = "Epstein-Barr virus LMP2A drives B cell development and survival in the absence of normal B cell receptor signals",

abstract = "Epstein-Barr virus (EBV) establishes a persistent latent infection in peripheral B lymphocytes in humans and is associated with a variety of malignancies and proliferative disorders. Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed in latently infected B lymphocytes in vivo. LMP2A blocks B cell receptor (BCR) signal transduction in vitro by binding the Syk and Lyn protein tyrosine kinases. To analyze the significance of LMP2A expression in vivo, transgenic mice with B cell lineage expression of LMP2A were generated. LMP2A expression results in the bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin- negative cells to colonize peripheral lymphoid organs, indicating that LMP2A possesses a constitutive signaling activity in nontransformed cells.",

author = "Caldwell, {Robert G.} and Wilson, {Joanna B.} and Anderson, {Steven J.} and Richard Longnecker",

note = "Funding Information: We thank Luz Longan for breeding and maintaining the transgenic animal colony and Lynn Doglio and the Northwestern University/CMIERTransgenic Facility for generating transgenic founder animals. R. G. C. is supported in part by the Northwestern University Carcinogenisis Training Grant 5T32CA09560 from the National Institutes of Health. S. J. A. is supported by Public Health Service grant GM55626. R. L. is supported by Public Health Service grants CA62234 and CA73507 from the National Cancer Institute. R. L. is a scholar of the Leukemia Society of America. ",

year = "1998",

month = sep,

doi = "10.1016/S1074-7613(00)80623-8",

language = "English (US)",

volume = "9",

pages = "405--411",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - Epstein-Barr virus LMP2A drives B cell development and survival in the absence of normal B cell receptor signals

AU - Caldwell, Robert G.

AU - Wilson, Joanna B.

AU - Anderson, Steven J.

AU - Longnecker, Richard

N1 - Funding Information: We thank Luz Longan for breeding and maintaining the transgenic animal colony and Lynn Doglio and the Northwestern University/CMIERTransgenic Facility for generating transgenic founder animals. R. G. C. is supported in part by the Northwestern University Carcinogenisis Training Grant 5T32CA09560 from the National Institutes of Health. S. J. A. is supported by Public Health Service grant GM55626. R. L. is supported by Public Health Service grants CA62234 and CA73507 from the National Cancer Institute. R. L. is a scholar of the Leukemia Society of America.

PY - 1998/9

Y1 - 1998/9

N2 - Epstein-Barr virus (EBV) establishes a persistent latent infection in peripheral B lymphocytes in humans and is associated with a variety of malignancies and proliferative disorders. Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed in latently infected B lymphocytes in vivo. LMP2A blocks B cell receptor (BCR) signal transduction in vitro by binding the Syk and Lyn protein tyrosine kinases. To analyze the significance of LMP2A expression in vivo, transgenic mice with B cell lineage expression of LMP2A were generated. LMP2A expression results in the bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin- negative cells to colonize peripheral lymphoid organs, indicating that LMP2A possesses a constitutive signaling activity in nontransformed cells.

AB - Epstein-Barr virus (EBV) establishes a persistent latent infection in peripheral B lymphocytes in humans and is associated with a variety of malignancies and proliferative disorders. Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed in latently infected B lymphocytes in vivo. LMP2A blocks B cell receptor (BCR) signal transduction in vitro by binding the Syk and Lyn protein tyrosine kinases. To analyze the significance of LMP2A expression in vivo, transgenic mice with B cell lineage expression of LMP2A were generated. LMP2A expression results in the bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin- negative cells to colonize peripheral lymphoid organs, indicating that LMP2A possesses a constitutive signaling activity in nontransformed cells.

UR - http://www.scopus.com/inward/record.url?scp=0032169313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032169313&partnerID=8YFLogxK

U2 - 10.1016/S1074-7613(00)80623-8

DO - 10.1016/S1074-7613(00)80623-8

M3 - Article

C2 - 9768760

AN - SCOPUS:0032169313

SN - 1074-7613

VL - 9

SP - 405

EP - 411

JO - Immunity

JF - Immunity

IS - 3

ER -

Epstein-Barr virus LMP2A drives B cell development and survival in the absence of normal B cell receptor signals

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this