TY - JOUR
T1 - Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates
AU - Quattrocelli, Mattia
AU - Giacomazzi, Giorgia
AU - Broeckx, Sarah Y.
AU - Ceelen, Liesbeth
AU - Bolca, Selin
AU - Spaas, Jan H.
AU - Sampaolesi, Maurilio
N1 - Funding Information:
This work has been funded by “ Opening The Future ” Campaign ( EJJ-OPTFUT-02010 ), CARE-MI FP7 , AFM , CARIPLO , FWO (#G060612N , #G0A8813N and #G088715N ), GOA , IUAP , and OT grants. M.Q. is supported by FWO (Postdoctoral Fellowship #1263314N ) and AFM (Trampoline Grant #18373 ). We are grateful to Michael David for technical help with the COMP qRT-PCR assay. We thank Christina Vochten and Vicky Raets for professional administrative assistance. We would also like to thank Paolo Luban and Rondoufonds voor Duchenne Onderzoek for kind donations. J.H.S. declares competing financial interests as shareholder in Global Stem Cell Technology (GST). S.Y.B. and J.H.S. are both employed by GST and are inventors of several pending patents owned by GST ( BE2012/0656 ; WO2014053418A9 ; WO2014053420A1 ; PCT/EP2013/075782 ).
PY - 2016/1/12
Y1 - 2016/1/12
N2 - Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open question is whether equine iPSCs differentiate toward muscle and cartilage, and whether donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs). Despite similar levels of pluripotency characteristics, the myogenic differentiation appeared enhanced in MAB-iPSCs. Conversely, the chondrogenic differentiation was augmented in MSC-iPSCs through both teratoma and in vitro differentiation assays. Thus, our data suggest that equine iPSCs can differentiate toward the myogenic and chondrogenic lineages, and can present a skewed differentiation potential in favor of the source cell lineage.
AB - Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open question is whether equine iPSCs differentiate toward muscle and cartilage, and whether donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs). Despite similar levels of pluripotency characteristics, the myogenic differentiation appeared enhanced in MAB-iPSCs. Conversely, the chondrogenic differentiation was augmented in MSC-iPSCs through both teratoma and in vitro differentiation assays. Thus, our data suggest that equine iPSCs can differentiate toward the myogenic and chondrogenic lineages, and can present a skewed differentiation potential in favor of the source cell lineage.
KW - chondrogenic differentiation
KW - equine iPSCs
KW - equine stem cells
KW - intrinsic lineage propensity
KW - myogenic differentiation
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U2 - 10.1016/j.stemcr.2015.12.005
DO - 10.1016/j.stemcr.2015.12.005
M3 - Article
C2 - 26771353
AN - SCOPUS:84955255165
VL - 6
SP - 55
EP - 63
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 1
ER -