ERBP, a novel estrogen receptor binding protein enhancing the activity of estrogen receptor

Hengfu Bu, Papreddy Kashireddy, Jeffrey Chang, Yiwei Tony Zhu, Zhongyi Zhang, Wei Zheng, Sambasiva Rao Musunuri, Yijun Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

To understand the mechanism by which estrogen receptor (ER) activates transcription in a tissue specific fashion, we isolated ERα binding protein (ERBP) by performing yeast two-hybrid screening with human mammary gland cDNA library. ERBP is a nuclear protein and its mRNA is ubiquitously expressed. The in vitro interaction of ERBP with ERα was demonstrated by GST pull-down assay and this interaction was enhanced by estrogen. In addition, ERBP also bound to PPARγ, RXRα, and ERβ. ERBP interacted with the DNA binding domain and the hinge region of ERα. There are two ERα binding regions on ERBP. The binding of ERBP region at C-terminus to ERα is increased by estrogen while the binding of ERBP region at N-terminus is not affected by estrogen. The interaction of ERBP with ERα was further confirmed in vivo by immunoprecipitation. Transient transfection experiment demonstrated that ERBP enhanced the transcriptional activity of ERα.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume317
Issue number1
DOIs
StatePublished - Apr 23 2004

Keywords

  • Coactivator
  • Estrogen receptor binding protein
  • Estrogen receptor α

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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