Erythropoietin-regulated oxidative stress negatively affects enucleation during terminal erythropoiesis

Baobing Zhao; Yang Mei; Jing Yang; Peng Ji

doi:10.1016/j.exphem.2016.06.249

Erythropoietin-regulated oxidative stress negatively affects enucleation during terminal erythropoiesis

Baobing Zhao, Yang Mei, Jing Yang, Peng Ji^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Differentiating erythroblasts are exposed to an oxidative environment. The dynamics of oxidative status during terminal erythropoiesis and how they affect cell differentiation in response to erythropoietin (Epo) are unclear. Here, we show that Epo induces reactive oxygen species (ROS) production in the early stages of terminal erythropoiesis. The levels of ROS correlate with CD71 surface expression and the uptake of iron and transferrin. ROS decreases in the late stages of terminal erythropoiesis, when the cells are preparing for enucleation. Consistently, treatment of erythroblasts with a low dose (5 mM) of N-acetyl-cysteine (NAC), a ROS scavenger, promotes enucleation. However, a high dose (20 mM) of NAC leads to significant cell death. Our study reveals an important function of Epo in regulating the dynamics of oxidative status and enucleation.

Original language	English (US)
Pages (from-to)	975-981
Number of pages	7
Journal	Experimental Hematology
Volume	44
Issue number	10
DOIs	https://doi.org/10.1016/j.exphem.2016.06.249
State	Published - Oct 1 2016

Funding

This study was supported by the National Institutes of Health (Grants R00 HL102154 and R01 DK102718 ) and the American Society of Hematology Scholar (awarded to P.J.).

ASJC Scopus subject areas

Molecular Biology
Hematology
Genetics
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.exphem.2016.06.249

Cite this

@article{44c44805ce934f72ac57985ee4b61d2c,

title = "Erythropoietin-regulated oxidative stress negatively affects enucleation during terminal erythropoiesis",

abstract = "Differentiating erythroblasts are exposed to an oxidative environment. The dynamics of oxidative status during terminal erythropoiesis and how they affect cell differentiation in response to erythropoietin (Epo) are unclear. Here, we show that Epo induces reactive oxygen species (ROS) production in the early stages of terminal erythropoiesis. The levels of ROS correlate with CD71 surface expression and the uptake of iron and transferrin. ROS decreases in the late stages of terminal erythropoiesis, when the cells are preparing for enucleation. Consistently, treatment of erythroblasts with a low dose (5 mM) of N-acetyl-cysteine (NAC), a ROS scavenger, promotes enucleation. However, a high dose (20 mM) of NAC leads to significant cell death. Our study reveals an important function of Epo in regulating the dynamics of oxidative status and enucleation.",

author = "Baobing Zhao and Yang Mei and Jing Yang and Peng Ji",

note = "Publisher Copyright: {\textcopyright} 2016 ISEH - International Society for Experimental Hematology",

year = "2016",

month = oct,

day = "1",

doi = "10.1016/j.exphem.2016.06.249",

language = "English (US)",

volume = "44",

pages = "975--981",

journal = "Experimental Hematology",

issn = "0301-472X",

publisher = "Elsevier Inc.",

number = "10",

}

TY - JOUR

T1 - Erythropoietin-regulated oxidative stress negatively affects enucleation during terminal erythropoiesis

AU - Zhao, Baobing

AU - Mei, Yang

AU - Yang, Jing

AU - Ji, Peng

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Differentiating erythroblasts are exposed to an oxidative environment. The dynamics of oxidative status during terminal erythropoiesis and how they affect cell differentiation in response to erythropoietin (Epo) are unclear. Here, we show that Epo induces reactive oxygen species (ROS) production in the early stages of terminal erythropoiesis. The levels of ROS correlate with CD71 surface expression and the uptake of iron and transferrin. ROS decreases in the late stages of terminal erythropoiesis, when the cells are preparing for enucleation. Consistently, treatment of erythroblasts with a low dose (5 mM) of N-acetyl-cysteine (NAC), a ROS scavenger, promotes enucleation. However, a high dose (20 mM) of NAC leads to significant cell death. Our study reveals an important function of Epo in regulating the dynamics of oxidative status and enucleation.

AB - Differentiating erythroblasts are exposed to an oxidative environment. The dynamics of oxidative status during terminal erythropoiesis and how they affect cell differentiation in response to erythropoietin (Epo) are unclear. Here, we show that Epo induces reactive oxygen species (ROS) production in the early stages of terminal erythropoiesis. The levels of ROS correlate with CD71 surface expression and the uptake of iron and transferrin. ROS decreases in the late stages of terminal erythropoiesis, when the cells are preparing for enucleation. Consistently, treatment of erythroblasts with a low dose (5 mM) of N-acetyl-cysteine (NAC), a ROS scavenger, promotes enucleation. However, a high dose (20 mM) of NAC leads to significant cell death. Our study reveals an important function of Epo in regulating the dynamics of oxidative status and enucleation.

UR - http://www.scopus.com/inward/record.url?scp=84989894978&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989894978&partnerID=8YFLogxK

U2 - 10.1016/j.exphem.2016.06.249

DO - 10.1016/j.exphem.2016.06.249

M3 - Article

C2 - 27364565

AN - SCOPUS:84989894978

SN - 0301-472X

VL - 44

SP - 975

EP - 981

JO - Experimental Hematology

JF - Experimental Hematology

IS - 10

ER -

Erythropoietin-regulated oxidative stress negatively affects enucleation during terminal erythropoiesis

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this