ESR study of the primary electron donor in highly 13C-enriched Chlorobium limicola f. thiosulfatophilum

Michael R. Wasielewski; Ursula H. Smith; James R. Norris

doi:10.1016/0014-5793(82)81089-2

ESR study of the primary electron donor in highly ¹³C-enriched Chlorobium limicola f. thiosulfatophilum

Michael R. Wasielewski^*, Ursula H. Smith, James R. Norris

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The photo-induced electron spin resonance signal of the primary donor P840⁺ in unit membrane fractions of the title photosynthetic bacterium was measured. Bacteria containing natural isotopic abundances and 82% ¹³C-enrichment were examined. Second moment analysis of the lineshape of the ¹³C-enriched P840⁺ signal shows that P840⁺ consists of two bacteriochlorophyll a-type macrocycles. The data thus obtained are independent of difficulties in interpreting either peak-to-peak linewidths or ENDOR data with respect to intermolecular spin delocalization.

Original language	English (US)
Pages (from-to)	138-140
Number of pages	3
Journal	FEBS Letters
Volume	149
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(82)81089-2
State	Published - Nov 22 1982

Keywords

Bacteriochlorophyll
Carbon 13 enrichment
Chlorobium limicola
ESR
Reaction center

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(82)81089-2

Cite this

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title = "ESR study of the primary electron donor in highly 13C-enriched Chlorobium limicola f. thiosulfatophilum",

abstract = "The photo-induced electron spin resonance signal of the primary donor P840+ in unit membrane fractions of the title photosynthetic bacterium was measured. Bacteria containing natural isotopic abundances and 82% 13C-enrichment were examined. Second moment analysis of the lineshape of the 13C-enriched P840+ signal shows that P840+ consists of two bacteriochlorophyll a-type macrocycles. The data thus obtained are independent of difficulties in interpreting either peak-to-peak linewidths or ENDOR data with respect to intermolecular spin delocalization.",

keywords = "Bacteriochlorophyll, Carbon 13 enrichment, Chlorobium limicola, ESR, Reaction center",

author = "Wasielewski, {Michael R.} and Smith, {Ursula H.} and Norris, {James R.}",

year = "1982",

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T1 - ESR study of the primary electron donor in highly 13C-enriched Chlorobium limicola f. thiosulfatophilum

AU - Wasielewski, Michael R.

AU - Smith, Ursula H.

AU - Norris, James R.

PY - 1982/11/22

Y1 - 1982/11/22

N2 - The photo-induced electron spin resonance signal of the primary donor P840+ in unit membrane fractions of the title photosynthetic bacterium was measured. Bacteria containing natural isotopic abundances and 82% 13C-enrichment were examined. Second moment analysis of the lineshape of the 13C-enriched P840+ signal shows that P840+ consists of two bacteriochlorophyll a-type macrocycles. The data thus obtained are independent of difficulties in interpreting either peak-to-peak linewidths or ENDOR data with respect to intermolecular spin delocalization.

AB - The photo-induced electron spin resonance signal of the primary donor P840+ in unit membrane fractions of the title photosynthetic bacterium was measured. Bacteria containing natural isotopic abundances and 82% 13C-enrichment were examined. Second moment analysis of the lineshape of the 13C-enriched P840+ signal shows that P840+ consists of two bacteriochlorophyll a-type macrocycles. The data thus obtained are independent of difficulties in interpreting either peak-to-peak linewidths or ENDOR data with respect to intermolecular spin delocalization.

KW - Bacteriochlorophyll

KW - Carbon 13 enrichment

KW - Chlorobium limicola

KW - ESR

KW - Reaction center

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