Abstract
Yeast enolase (EC 4.2.1.11) is rapidly inactivated at pH 6.1 by three different water-soluble carbodiimides - 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride, 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate, and 1-ethyl-3-(4-azonia-4,4-dimethylpentyl)-carbodiimide iodide. Inactivation is most likely due to the modification of essential carboxyl residues at the enzyme active site.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 59-65 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 87 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 15 1979 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
}
Essential carboxyl residues in yeast enolase. / George, Alfred L.; Borders, C. L.
In: Biochemical and Biophysical Research Communications, Vol. 87, No. 1, 15.03.1979, p. 59-65.Research output: Contribution to journal › Article
TY - JOUR
T1 - Essential carboxyl residues in yeast enolase
AU - George, Alfred L.
AU - Borders, C. L.
PY - 1979/3/15
Y1 - 1979/3/15
N2 - Yeast enolase (EC 4.2.1.11) is rapidly inactivated at pH 6.1 by three different water-soluble carbodiimides - 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride, 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate, and 1-ethyl-3-(4-azonia-4,4-dimethylpentyl)-carbodiimide iodide. Inactivation is most likely due to the modification of essential carboxyl residues at the enzyme active site.
AB - Yeast enolase (EC 4.2.1.11) is rapidly inactivated at pH 6.1 by three different water-soluble carbodiimides - 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride, 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate, and 1-ethyl-3-(4-azonia-4,4-dimethylpentyl)-carbodiimide iodide. Inactivation is most likely due to the modification of essential carboxyl residues at the enzyme active site.
UR - http://www.scopus.com/inward/record.url?scp=0018791116&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018791116&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(79)91646-2
DO - 10.1016/0006-291X(79)91646-2
M3 - Article
VL - 87
SP - 59
EP - 65
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -