Abstract
Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Patients and Methods: Cancer cells, isolated from pleural effusion using a gradient method, were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in the culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining the neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. Neither EGFR or KRAS mutation were found. Conclusion: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing the metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
Original language | English (US) |
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Pages (from-to) | 2821-2827 |
Number of pages | 7 |
Journal | Anticancer research |
Volume | 26 |
Issue number | 4 B |
State | Published - Jul 2006 |
Keywords
- Amylase
- Cell line
- IMEC-2
- Lung cancer
- Sialylation
ASJC Scopus subject areas
- Oncology
- Cancer Research